Antioxidant activity of betalains from plants of the Amaranthaceae
Antioxidant activity of betalains from plants of the Amaranthaceae
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DOI：
10.1021/jf030045u
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发表时间：
2003-04-09
影响因子：
6.1
通讯作者：
Corke, H
中科院分区：
文献类型：
--
作者：
Cai, YZ;Sun, M;Corke, H
采用改进的DPPH法对苋科植物甜菜红色素（7个纯品和4个合并组分）的抗氧化活性进行了评价。（1，1-二苯基-2-苦肼基）法。所有测试的甜菜色素都表现出很强的抗氧化活性。它们的EC 50值范围为3.4 - 8.4 μ M。Gomphrenin型betacyanins（平均值= 3.7 μ M）和betaxanthins（平均值= 4.2 μ M）表现出最强的抗氧化活性，比抗坏血酸（13.9 μ M）强3-4倍，也比芦丁（6.1 μ M）和儿茶素（7.2 μ M）强。甜菜色素的抗氧化活性按以下顺序降低：简单的高铁黄素>酰化高铁黄素>多巴胺-甜菜黄素>（S）-半胱氨酸-甜菜黄素> 3-甲氧基酪胺-甜菜黄素>甜菜碱/异甜菜碱>青蒜素> Iresinins >苋菜红/异苋菜红。本研究还对甜菜色素的化学结构与活性之间的关系进行了探讨。甜菜色素的自由基清除活性通常随着羟基/亚氨基的数目而增加，并且取决于甜菜色素分子中羟基的位置和糖苷配基的糖基化。
Antioxidant activity of betalain pigments (seven pure compounds and four combined fractions) from plants of the family Amaranthaceae was evaluated using the modified DPPH. (1,1-diphenyl-2-picrylhydrazyl) method. All tested betalains exhibited strong antioxidant activity. Their EC50 values ranged from 3.4 to 8.4 muM. Gomphrenin type betacyanins (mean = 3.7 muM) and betaxanthins (mean = 4.2 muM) demonstrated the strongest antioxidant activity, 3-4-fold stronger than ascorbic acid (13.9 muM) and also stronger than rutin (6.1 muM) and catechin (7.2 muM). Antioxidant activity of the tested betalains decreased in the following order: simple gomphrenins > acylated gomphrenins > dopamine-betaxanthin > (S)-tryptophan-betaxanthin > 3-methoxytyramine-betaxanthin > betanin/isobetanin > celosianins > iresinins > amaranthine/isoamaranthine. This study also investigated and discussed the relationship between the chemical structure and the activity of the betalains. The free radical scavenging activity of the betalains usually increased with the numbers of hydroxyl/imino groups and, moreover, depended on the position of hydroxyl groups and glycosylation of aglycones in the betalain molecules.