Calcium signalling in and around the nuclear envelope
Calcium signalling in and around the nuclear envelope
复制标题
DOI：
10.1042/bst0310076
复制
发表时间：
2003-02-01
影响因子：
3.9
通讯作者：
Gerasimenko, O
中科院分区：
文献类型：
--
作者：
Gerasimenko, J;Maruyama, Y;Gerasimenko, O
我们比较了Ins（1，4，5）P-3（IP 3）、cADP-核糖（cADPR）和烟酸-腺苷二核苷酸磷酸（NAADP）从离体细胞核包膜的钙动员与完整的离体胰腺腺泡细胞中的钙信号。用钙敏感的荧光探针研究了Ca 2+的摄取和释放。在目前的研究中，我们已经表明，所有的钙信使诱导Ca 2+从核膜释放。用毒胡萝卜素预处理细胞核完全取消了对钙信使的反应，表明在分离的细胞核中的Ca 2+储存是毒胡萝卜素敏感的。使用不同的药理学工具，我们表明，Ca 2+从胰腺细胞核释放是不太可能发生的存储器以外的内质网特征。我们的结论是，所有三个钙信使可以释放Ca 2+从胰腺腺泡核商店，如先前所示的IP 3和cADPR。看来NAADP从具有内质网特征的相同的IP 3-和cADPR-敏感性储存释放Ca 2+。
We have compared calcium mobilization by Ins(1,4,5)P-3 (IP3), cADP-ribose (cADPR) and nicotinic acid-adenosine dinucleotide phosphate (NAADP) from the envelope of isolated nuclei with the calcium signalling in intact isolated pancreatic acinar cells. Ca2+ uptake and release were studied with calcium-sensitive fluorescent probes. In the present study, we have shown that all calcium messengers induce Ca2+ release from the nuclear envelope. Pre-treatment of nuclei with thapsigargin completely abolished the responses to the calcium messengers, indicating that Ca2+ stores in isolated nuclei are thapsigargin-sensitive. Using different pharmacological tools, we show that Ca2+ release from pancreatic nuclei is unlikely to occur from stores other than those with endoplasmic reticulum characteristics. We conclude that all three calcium messengers can release Ca2+ from pancreatic acinar nuclear stores, as previously shown for IP3 and cADPR. it would appear that NAADP releases Ca2+ from the same IP3- and cADPR-sensitive stores with endoplasmic reticulum characteristics.