The genome of opportunistic fungal pathogen Fusarium oxysporum carries a unique set of lineage-specific chromosomes
The genome of opportunistic fungal pathogen Fusarium oxysporum carries a unique set of lineage-specific chromosomes
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DOI：
10.1038/s42003-020-0770-2
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发表时间：
2020-01-31
影响因子：
5.9
通讯作者：
Ma, Li-Jun
中科院分区：
文献类型：
--
作者：
Zhang, Yong;Yang, He;Ma, Li-Jun
尖孢镰刀菌是一种跨界真菌病原体，感染植物和人类。水平转移的谱系特异性（LS）染色体被报道用于确定植物病原F.尖孢菌属然而，LS染色体在人类致病菌株中的存在和功能重要性尚不清楚。在这里，我们报告四个独特的LS染色体在人类致病株NRRL 32931，从白血病患者分离。这些LS染色体没有管家基因，但显着丰富的基因编码金属离子转运蛋白和阳离子转运蛋白。NRRL 32931 LS基因的同源物，包括血浆铜蓝蛋白的同源物和有助于碱性pH响应性转录因子PacC/Rim 1 p扩增的基因，也存在于NRRL 47514的基因组中，NRRL 47514是一种与镰刀菌角膜炎爆发相关的菌株。这项研究提供了第一个证据，据我们所知，在两个人类致病真菌基因组的基因组区室化，并建议在生态位适应LS染色体的重要作用。Zhang，Yang等人比较了临床获得的尖孢镰刀菌分离株与植物病原菌株，以检查在确定宿主特异性时谱系特异性染色体的转移。他们发现了四个独特的谱系特异性染色体，似乎有助于真菌适应人类宿主。
Fusarium oxysporum is a cross-kingdom fungal pathogen that infects plants and humans. Horizontally transferred lineage-specific (LS) chromosomes were reported to determine host-specific pathogenicity among phytopathogenic F. oxysporum. However, the existence and functional importance of LS chromosomes among human pathogenic isolates are unknown. Here we report four unique LS chromosomes in a human pathogenic strain NRRL 32931, isolated from a leukemia patient. These LS chromosomes were devoid of housekeeping genes, but were significantly enriched in genes encoding metal ion transporters and cation transporters. Homologs of NRRL 32931 LS genes, including a homolog of ceruloplasmin and the genes that contribute to the expansion of the alkaline pH-responsive transcription factor PacC/Rim1p, were also present in the genome of NRRL 47514, a strain associated with Fusarium keratitis outbreak. This study provides the first evidence, to our knowledge, for genomic compartmentalization in two human pathogenic fungal genomes and suggests an important role of LS chromosomes in niche adaptation. Zhang, Yang et al. compare a Fusarium oxysporum isolate obtained clinically to a phytopathogenic strain to examine transfer of lineage-specific chromosomes in determining host specificity. They find four unique lineage-specific chromosomes that seem to contribute to fungal adaptation to human hosts.