Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome.
Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome.
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DOI：
10.1111/j.1574-6976.2008.00141.x
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发表时间：
2009-01
影响因子：
11.3
通讯作者：
Emili A
中科院分区：
文献类型：
--
作者：
Díaz-Mejía JJ;Babu M;Emili A
细菌细胞包膜由脂质、蛋白质和碳水化合物的复杂排列组成，作为微生物与其环境或病原体与人类宿主之间的界面。大肠杆菌长期以来一直被研究作为一个领先的模式系统，以阐明微生物细胞被膜生物学的基本机制。这包括广泛的描述的分子身份，生化活动和进化轨迹的完整的跨膜蛋白，其中许多发挥关键作用的传染病和抗生素耐药性。然而，令人惊讶的是，只有一半的C。1200个E.目前，大肠杆菌已经通过实验确定了功能，这表明有机会发现。在这篇综述中，我们总结了最先进的计算和蛋白质组学方法来确定的组成部分，E。大肠杆菌细胞包膜蛋白质组，以及探索物理和功能的相互作用，其生物起源和功能。我们还提供了一个全面的比较基准分析不同的生物信息学和蛋白质组学方法的性能，通常用于确定细菌蛋白质的亚细胞定位。
The bacterial cell-envelope consists of a complex arrangement of lipids, proteins and carbohydrates that serves as the interface between a microorganism and its environment or, with pathogens, a human host. Escherichia coli has long been investigated as a leading model system to elucidate the fundamental mechanisms underlying microbial cell-envelope biology. This includes extensive descriptions of the molecular identities, biochemical activities and evolutionary trajectories of integral transmembrane proteins, many of which play critical roles in infectious disease and antibiotic resistance. Strikingly, however, only half of the c. 1200 putative cell-envelope-related proteins of E. coli currently have experimentally attributed functions, indicating an opportunity for discovery. In this review, we summarize the state of the art of computational and proteomic approaches for determining the components of the E. coli cell-envelope proteome, as well as exploring the physical and functional interactions that underlie its biogenesis and functionality. We also provide a comprehensive comparative benchmarking analysis on the performance of different bioinformatic and proteomic methods commonly used to determine the subcellular localization of bacterial proteins.
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影响因子：
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