CONSTRUCTION OF A TETRACYCLINE-INDUCIBLE PROMOTER IN SCHIZOSACCHAROMYCES-POMBE
CONSTRUCTION OF A TETRACYCLINE-INDUCIBLE PROMOTER IN SCHIZOSACCHAROMYCES-POMBE
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DOI：
10.1007/bf00351693
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发表时间：
1992-04-01
期刊：
影响因子：
2.5
通讯作者：
GATZ, C
中科院分区：
文献类型：
--
作者：
FARYAR, K;GATZ, C
We have developed a tightly repressed Schizosaccharomyces pombe promoter which can be efficiently induced by Tetracycline. This promoter is a derivative of the plant viral cauliflower mosaic virus 35S promoter which normally functions as a strong constitutive promoter in S. pombe. Location of three binding sites for the Tn10-encoded Tet repressor in the vicinity of the TATA-box of the CaMV 35S promoter led to a tight repression of promoter activity in the presence of the Tet repressor protein. Up to a 400-fold induction was observed after addition of the inducer Tetracycline, which inactivates the operator-binding capacity of the repressor.
