Poly (dopamine) coated superparamagnetic iron oxide nanocluster for noninvasive labeling, tracking, and targeted delivery of adipose tissue-derived stem cells.
Poly (dopamine) coated superparamagnetic iron oxide nanocluster for noninvasive labeling, tracking, and targeted delivery of adipose tissue-derived stem cells.
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DOI：
10.1038/srep18746
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发表时间：
2016-01-05
影响因子：
4.6
通讯作者：
Liu J
中科院分区：
文献类型：
--
作者：
Liao N;Wu M;Pan F;Lin J;Li Z;Zhang D;Wang Y;Zheng Y;Peng J;Liu X;Liu J
移植后体内细胞的跟踪和监测可以为干细胞治疗提供关键信息。磁共振成像（MRI）结合造影剂被认为是一种有效的和非侵入性的技术，在活体细胞跟踪。然而，商业超顺磁性氧化铁纳米粒子（SPION）应用于标记细胞遭受的缺点，如潜在的毒性，低标记效率，低对比度增强。在此，脂肪组织来源的干细胞（ADSC）被有效地用聚（多巴胺）包被的SPION（SPION簇@PDA）标记，而不影响其活力、增殖、凋亡、表面标志物表达以及其自我更新能力和多分化潜能。尾静脉注射标记细胞后，四氯化碳诱导的损伤肝脏MRI信号呈负性增强，SPIONs簇@PDA标记的ADSCs对损伤肝脏具有良好的修复作用。此外，在切除皮肤创伤小鼠模型中，通过使用外部磁场，可以实现增强SPIONs簇@ PDA标记的ADSCs对感兴趣组织的靶向归巢和修复效果。因此，我们提供了一种简便、安全、无创和敏感的方法，用于体外磁场靶向递送和基于MRI的体内移植细胞跟踪。
Tracking and monitoring of cells in vivo after transplantation can provide crucial information for stem cell therapy. Magnetic resonance imaging (MRI) combined with contrast agents is believed to be an effective and non-invasive technique for cell tracking in living bodies. However, commercial superparamagnetic iron oxide nanoparticles (SPIONs) applied to label cells suffer from shortages such as potential toxicity, low labeling efficiency, and low contrast enhancing. Herein, the adipose tissue-derived stem cells (ADSCs) were efficiently labeled with SPIONs coated with poly (dopamine) (SPIONs cluster@PDA), without affecting their viability, proliferation, apoptosis, surface marker expression, as well as their self-renew ability and multi-differentiation potential. The labeled cells transplanted into the mice through tail intravenous injection exhibited a negative enhancement of the MRI signal in the damaged liver-induced by carbon tetrachloride, and subsequently these homed ADSCs with SPIONs cluster@PDA labeling exhibited excellent repair effects to the damaged liver. Moreover, the enhanced target-homing to tissue of interest and repair effects of SPIONs cluster@PDA-labeled ADSCs could be achieved by use of external magnetic field in the excisional skin wound mice model. Therefore, we provide a facile, safe, noninvasive and sensitive method for external magnetic field targeted delivery and MRI based tracking of transplanted cells in vivo.