MECHANISM OF MITOCHONDRIAL SWELLING
MECHANISM OF MITOCHONDRIAL SWELLING
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DOI：
10.1073/pnas.58.2.612
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发表时间：
1967-01-01
影响因子：
11.1
通讯作者：
GREEN, DE
中科院分区：
文献类型：
--
作者：
BLONDIN, GA;GREEN, DE
线粒体在各种条件下膨胀的能力是十多年来深入研究的主题。线粒体肿胀最令人困惑的特征在于它可以在渗透压差异很大的介质中发生。线粒体在十分之一摩尔蔗糖溶液中的溶胀通常不会发生，除非与能量相关的功能偶联; 4-6当通过添加适当的抑制剂抑制这种通电溶胀时，溶胀被逆转。然而，在蒸馏水和碱金属氯化物的十分之一摩尔溶液中，线粒体体积和能量状态的产生之间的反向关系已被发现在某些条件下占主导地位，即，通过与能量相关功能的偶联，溶胀被逆转。这些和许多其他观察结果尖锐地指出了以前由Pressman ′ 3 14和Lardy ′ 5所强调的事实，即仅用渗透现象来解释线粒体体积的变化是远远不够的。我们实验室积累的数据为线粒体肿胀的根本不同的解释提供了基础。线粒体膜的超微结构变化似乎是肿胀问题的核心。本文是有关线粒体肿胀与离子诱导的线粒体内膜超微结构重排的实验基础的解释性报道。线粒体内膜肿胀的一种表现。在碱金属盐溶液中的十摩尔溶胀”6（以及在蒸馏水中的溶胀“7 -19）可以统一描述为牛心线粒体内膜的以下超微结构变化。随着肿胀的进行，管状嵴收缩并最终消失。重复单元从嵴中抽出，进入内膜的囊状部分（见图1）。因此，当嵴被拉回到外周膜中时，膜的直径扩大。
The ability of mitochondria to swell under a wide variety of conditions is a property which has been the subject of intensive investigation for more than a decade. 1 The most baffling feature of mitochondrial swelling lies in the fact that it can take place in media of widely different osmolarities. Swelling of mitochonldria in decimolar sucrose solutions generally does not occur unless coupled to energy-linked functions; 4-6 when such energized swelling is suppressed by addition of appropriate inhibitors, swelling is reversed. 7-'However, the reverse relationship between mitochondrial volume and the generation of the energized state has been found to prevail under certain conditions in distilled water and in decimolar solutions of alkali metal chlorides;'0-12 ie, swelling is reversed by coupling to energy-linked functions. These and many otherobservations sharply point up the fact stressed previously by Pressman'3 14 and Lardy'5 that an explanation of changes in mito-chondrial volume exclusively in terms of osmotic phenomena is far from adequate. Data have beenaccumulated in our laboratory which provide the foundation for a radicallydifferent interpretation of mitochondrial swelling. Ultrastructural changes in the mitochondrial membrane appearto be central to the problem of swelling. The present communication is an interpretative account of the investiga-tions which haveprovided the experimental foundations for relating mitochondrial swelling to ion-induced, ultrastructural rearrangement of the inner mitochondrial membrane.Swelling as an Expression of the InneriMitochondrial M11emnbrane. Swelling in solutions decimolar in alkali-metal salts" 6 (and also in distilled water'7-19) can be uniformly described in terms of the following ultrastructural changes in the inner membrane of beef heart mitochondria. As swelling proceeds, the tubular cristae are retracted and eventually disappear. The repeating units are withdrawn from the cristae and paid into the vesicular part of the inner membrane (see Fig. 1). Thus, as thecristae are pulled back into the peripheral membrane, the membrane expands in diameter.