Surface-Imprinted Nanoparticles Prepared with a His-Tag-Anchored Epitope as the Template
Surface-Imprinted Nanoparticles Prepared with a His-Tag-Anchored Epitope as the Template
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以组氨酸标签锚定表位为模板制备表面印迹纳米粒子
DOI：
10.1021/ac5047246
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发表时间：
2015
影响因子：
7.4
通讯作者：
Zhang Yukui
中科院分区：
文献类型：
--
作者：
Li Senwu;Yang Kaiguang;Liu Jianxi;Jiang Bo;Zhang Lihua;Zhang Yukui
人工抗体对生物分子的特异性识别激发了化学家和生物学家的兴趣。在此，我们提出了一种新的方法来制备具有高模板利用率的表位取向表面印迹纳米颗粒。利用His-tag作为锚点，促进表位的固定/移除和多巴胺的自聚合来控制印迹壳的厚度，制备的表位印迹纳米颗粒对目标蛋白具有特异性识别。此外，由于his标签锚定表位的亲水性得到改善，该方法为印迹不同极性的表位开辟了一条通用途径。
The specific recognition of biomolecules by artificial antibodies has inspired fascination among chemists and biologists. Herein, we propose a new method to prepare epitope-oriented surface-imprinted nanoparticles with high template utilization efficiency. Using a His-tag as the anchor to facilitate the epitope immobilization/removal and the self-polymerization of dopamine to control the imprinted shell thickness, the prepared epitope-imprinted nanoparticles show specific recognition of the target protein. Moreover, with improved hydrophilicity of the His-tag-anchored epitope, this method opens up a universal route for imprinting epitopes with various polarities.