Heterogeneity of adipose stromal vascular fraction cells from the different harvesting sites in rats
Heterogeneity of adipose stromal vascular fraction cells from the different harvesting sites in rats
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DOI：
10.1002/ar.24915
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发表时间：
2022-03
期刊：
影响因子：
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通讯作者：
H. Hendawy;M. Kaneda;T. Yoshida;Elsayed Metwally;Lina Hambe;T. Yoshida;K. Shimada;R. Tanaka
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文献类型：
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作者：
H. Hendawy;M. Kaneda;T. Yoshida;Elsayed Metwally;Lina Hambe;T. Yoshida;K. Shimada;R. Tanaka
在兽医和人类健康方面，再生医学为各种疾病提供了有希望的治疗方法。再生医学中的限速挑战之一是在培养中扩增和生长细胞所需的大量时间和技术。因此，基质血管部分（SVF）显示了各种细胞治疗方法的重要前景。本研究旨在确定和研究从雌性Sprague-Dawley（S.D.）大鼠首先，使用苏木精和伊红（H&E）分析来自卵巢、肾、肠系膜和网膜部位的AT样本的形态学变化。采用免疫组化法检测CD 34阳性基质细胞的存在。然后，检查分离的SVF细胞的细胞活力和细胞产量差异。最后，用流式细胞仪检测SVF细胞亚群中间充质干细胞和造血标志物的表达。还评价了多能基因表达谱。大网膜AT的CD 34染色明显高于其他解剖部位。尽管有最少的脂肪，网膜AT具有最高的SVF细胞分数和活细胞。沿着CD 90和CD 44的高表达，Oct 4、Sox 2和雷克斯-1基因水平在从网膜AT分离的SVF细胞中更高。总之，网膜脂肪是女性S.D.中SVF细胞分离的最佳候选物。具有最高SVF细胞分数的大鼠具有更高的MSC表型和多能性基因表达。
In both veterinary and human health, regenerative medicine offers a promising cure for various disorders. One of the rate‐limiting challenges in regenerative medicine is the considerable time and technique required to expand and grow cells in culture. Therefore, the stromal vascular fraction (SVF) shows a significant promise for various cell therapy approaches. The present study aimed to define and investigate the optimal harvest site of freshly isolated SVF cells from various adipose tissue (AT) depot sites in the female Sprague–Dawley (S.D.) rat. First, hematoxylin and eosin (H&E) were used to analyze the morphological variations in AT samples from peri‐ovarian, peri‐renal, mesenteric, and omental sites. The presence of putative stromal cells positive CD34 was detected using immunohistochemistry. Then, the isolated SVF cells were examined for cell viability and cellular yield differences. Finally, the expression of mesenchymal stem cells and hematopoietic markers in the SVF cells subpopulation was studied using flow cytometry. The pluripotent gene expression profile was also evaluated. CD34 staining of the omental AT was substantially higher than those of other anatomical sites. Despite having the least quantity of fat, omental AT has the highest SVF cell fraction and viable cells. Along with CD90 and CD44 higher expression, Oct4, Sox2, and Rex‐1 genes levels were higher in SVF cells isolated from the omental AT. To conclude, omental fat is the best candidate for SVF cell isolation in female S.D. rats with the highest SVF cell fraction with higher MSCs phenotypes and pluripotency gene expression.