Identification of Campylobacter jejuni genes involved in commensal colonization of the chick gastrointestinal tract
Identification of Campylobacter jejuni genes involved in commensal colonization of the chick gastrointestinal tract
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DOI：
10.1111/j.1365-2958.2004.03988.x
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发表时间：
2004-04-01
影响因子：
3.6
通讯作者：
DiRita, VJ
中科院分区：
文献类型：
--
作者：
Hendrixson, DR;DiRita, VJ
空肠弯曲菌是世界上发达国家人类细菌性胃肠炎的主要病因。这种细菌经常在包括鸟类在内的许多动物的胃肠道中促进肠道生活方式，食用或处理家禽肉类是C。空肠感染人类。为了了解该细菌如何促进共栖，我们使用标记标签的转座子突变并鉴定了代表C的22个不同基因的29个突变体。空肠菌株81-176参与鸡胃肠道的定殖。其中确定的决定因素是两个相邻的基因，一个编码甲基接受趋化蛋白（MCP），大概需要适当的趋化性，以特定的环境成分，和另一个基因编码一个假定的细胞色素c过氧化物酶，可能会起到减少周质过氧化氢应力在体内生长。任一基因的缺失导致整个胃肠道的生长减弱。进一步研究了10种其他推定的C.空肠揭示了盲肠定殖的野生型水平所需的另一个。这项研究代表了第一个遗传筛选，重点是在脊椎动物宿主中促进嗜盐性所必需的细菌要求。
Campylobacter jejuni is the leading cause of bacterial gastroenteritis in humans in developed countries throughout the world. This bacterium frequently promotes a commensal lifestyle in the gastrointestinal tracts of many animals including birds and consumption or handling of poultry meats is a prevalent source of C. jejuni for infection in humans. To understand how the bacterium promotes commensalism, we used signature-tagged transposon mutagenesis and identified 29 mutants representing 22 different genes of C. jejuni strain 81-176 involved in colonization of the chick gastrointestinal tract. Among the determinants identified were two adjacent genes, one encoding a methyl-accepting chemotaxis protein (MCP), presumably required for proper chemotaxis to a specific environmental component, and another gene encoding a putative cytochrome c peroxidase that may function to reduce periplasmic hydrogen peroxide stress during in vivo growth. Deletion of either gene resulted in attenuation for growth throughout the gastrointestinal tract. Further examination of 10 other putative MCPs or MCP-domain containing proteins of C. jejuni revealed one other required for wild-type levels of caecal colonization. This study represents one of the first genetic screens focusing on the bacterial requirements necessary for promoting commensalism in a vertebrate host.