Robust sealing of blood vessels with cholesteryl group-modified, Alaska pollock-derived gelatin-based biodegradable sealant under wet conditions
Robust sealing of blood vessels with cholesteryl group-modified, Alaska pollock-derived gelatin-based biodegradable sealant under wet conditions
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在潮湿条件下，使用胆固醇基团改性、阿拉斯加鳕鱼衍生的明胶基可生物降解密封剂牢固密封血管
DOI：
10.1166/jbn.2016.2210
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发表时间：
2016
影响因子：
2.9
通讯作者：
K.; Kajiyama
中科院分区：
文献类型：
--
作者：
Tetsushi T.; Mizuta;R.; Ito;T.; Yoshizawa;K.; Kajiyama
以胆固醇基修饰鳕鱼明胶衍生物（Chol-apGltn）的氨基，并与交联剂季戊四醇聚乙二醇醚四琥珀酰亚胺戊二酸酯（4S-PEG）结合，制备了一种新型外科封闭剂。使用新鲜猪主动脉作为粘附体测试所得密封剂的破裂强度。Chol-apGltn/4S-PEG密封剂在25.4 ± 2.0 s内固化。破裂强度随着Chol改性程度的增加而增加，最大值为8.3 mol%（8.3Chol-apGltn）。8.3Chol-apGltn/4S-PEG封闭剂的最高破裂强度为341.3 ± 77.5 mmHg，分别是原始apGltn/4S-PEG和商业纤维蛋白封闭剂的3.5倍和11.6倍。8.3Chol-apGltn/4S-PEG密封剂在溶液中仅轻微溶胀（与商业制备的密封剂相比为1.1倍）。此外，在植入4-8周后，观察到组织迁移到8.3Chol-apGltn/4S-PEG密封剂中，随后发生生物降解。这些结果表明，8.3Chol-apGltn/4S-PEG密封剂具有生物医学应用，包括用于心血管和胸外科手术。
A novel surgical sealant was developed by partially modifying the amino groups of Alaska pollock-derived gelatin derivative with cholesteryl groups (Chol-apGltn), which was combined with the crosslinker, pentaerythritol poly(ethylene glycol) ether tetrasuccinimidyl glutarate (4S-PEG). The burst strength of the resultant sealant was tested using fresh porcine aorta as an adherend. The Chol-apGltn/4S-PEG sealant cured within 25.4 ± 2.0 s. Burst strength increased with increasing degree of Chol modification up to a maximum value of 8.3 mol% (8.3Chol-apGltn). The highest burst strength of the 8.3Chol-apGltn/4S-PEG sealant was 341.3 ± 77.5 mmHg, which was 3.5- and 11.6-fold higher than that of the original apGltn/4S-PEG and commercial fibrin sealants, respectively. The 8.3Chol-apGltn/4S-PEG sealant swelled only slightly in solution (1.1-fold as compared to commercially prepared sealant). Furthermore, tissue migration into the 8.3Chol-apGltn/4S-PEG sealant and subsequent biodegradation was observed following implantation for 4–8 weeks. These results suggest that the 8.3Chol-apGltn/4S-PEG sealant has biomedical applications, including use in cardiovascular and thoracic surgery.