MAPPING STUDY OF NORADRENERGIC STIMULATION OF VASOPRESSIN RELEASE
MAPPING STUDY OF NORADRENERGIC STIMULATION OF VASOPRESSIN RELEASE
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DOI：
10.1016/0014-4886(90)90042-q
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发表时间：
1990-12-01
影响因子：
5.3
通讯作者：
SLADEK, CD
中科院分区：
文献类型：
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作者：
LEIBOWITZ, SF;EIDELMAN, D;SLADEK, CD
下丘脑去甲肾上腺素 (NE) 在控制加压素 (AVP) 释放中的确切作用仍不清楚，因为有关 NE 的抑制和兴奋作用的报道以及只有少数直接操作下丘脑的研究。本研究利用长期植入的旋转脑插管，在不受干扰和自由行为的大鼠中研究急性下丘脑输注单胺的影响，并比较了六个插管的反应性。结果表明，中枢去甲肾上腺素能刺激对血清 AVP 的兴奋作用具有高度的位点特异性，局限于室旁核 (PVN) 和视上核 (SON)。这两个细胞核似乎对 NE 输注有同样的反应，使血清 AVP 比基线水平升高三倍。相反，背内侧核中的NE产生的AVP增加明显较小，并且在腹内侧核、下丘脑后部或下丘脑外侧角周围没有观察到反应。在 PVN 中进行的进一步测试表明，该细胞核对 NE 输注有剂量依赖性反应。相比之下，在类似的测试条件下，多巴胺在相对高剂量下仅引起 AVP 的小幅增加，而 PVN 注射血清素则没有产生任何反应。这些结果支持了控制 AVP 释放的兴奋性去甲肾上腺素能系统的存在，并具体证明了 NE 的这种功能与其他下丘脑区域相比，位于 PVN 和 SON，并且在一定程度上被多巴胺模仿，但不是被血清素模仿。
The precise role of hypothalamic norepinephrine (NE) in the control of vasopressin (AVP) release has remained unclear, due to reports of both inhibitory and excitatory effects of NE and only a few studies with direct hypothalamic manipulations. The present study utilized a chronically implanted swivel brain cannula to investigate, in indisturbed and freely behaving rats, the impact of acute hypothalamic infusions of monoamines and compared the responsiveness of six cannula. Results indicated that the excitatory effect of central noradrenergic stimulation on serum AVP is highly site specific, localized to the paraventricular (PVN) and supraoptic (SON) nuclei. These two nuclei appeared to be equally responsive to NE infusion, yielding a threefold rise in serum AVP over baseline levels. In contrast, NE in the dorsomedial nucleus produced a significantly smaller increase in AVP, and no response was observed in the ventromedial nucleus, posterior hypothalamus, or perifornical lateral hypothalamus. Further tests conducted in the PVN showed this nucleus to respond in a dose-dependent manner to NE infusion. In contrast, under similar test conditions, dopamine caused only a small increase in AVP at a relatively high dose, while a PVN injection of serotonin produced no response. These results support the existence of an excitatory noradrenergic system controlling AVP release and specifically demonstrated that this function of NE is localized to the PVN and SON, in contrast to other hypothalamic areas, and is mimicked to some extent by dopamine but not by serotonin.