ID: 217
ID: 217
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编号：217
DOI：
10.1016/j.cyto.2015.08.221
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发表时间：
2015
期刊：
影响因子：
3.8
通讯作者：
Giotis E
中科院分区：
文献类型：
--
作者：
Giotis E
鸡肉几乎被普遍接受，不久将成为发达国家和发展中国家消费最多的肉类。鸡的病毒，无论是地方性的(如新城疫、马雷克或传染性法氏囊病病毒或传染性支气管炎病毒)，还是人畜共患病(特别是H5N1和H7N9等禽流感病毒)，都对这种全球重要的蛋白质来源的供应构成重大威胁。了解鸡先天反应的本质将有助于我们更好地了解这些病毒、它们的进化和致病机制，并可能帮助我们开发更有效的控制它们的方法。虽然首先在鸡细胞中发现了干扰素，但鸡干扰素反应的细节才刚刚被阐明，因为鸡干扰素是在1994年才被克隆出来的，鸡的基因组序列直到2004年才被报道。值得注意的是，干扰素刺激基因的特性在哺乳动物中得到了更好的描述。虽然关于鸡对病毒感染的反应的报道越来越多，但所描述的大多数调控基因都参与了病毒复制所需的细胞周期、代谢途径和细胞结构。我们进行了一项研究，目的是描述潜在的抗病毒基因的互补，在一个共同的研究和疫苗生产底物，即原代鸡胚成纤维细胞中鉴定重组I型干扰素诱导的基因。结果是可靠的，因为193个诱导基因中的155个是通过所使用的三种转录技术中的至少两种检测到的：Illumina RNA-seq、Affymetrix 32K基因芯片鸡基因组阵列和鸡基因1.0 ST阵列。
Chicken, which is almost universally-acceptable, will shortly become the most consumed meat in the developed and developing world. Viruses of chickens, whether enzootic (e.g. Newcastle, Mareks or infectious bursal disease viruses or infectious bronchitis virus) or zoonotic (especially avian influenza viruses such as H5N1 and H7N9), pose major threats to the supply of this globally important source of protein. Understanding the nature of the chicken innate responses will help us better understand these viruses, their evolution and their pathogenesis, and may help us develop more effective methods of controlling them. Although first discovered in chicken cells, details of the chicken interferon response are only just being elucidated, because chicken interferon was only cloned in 1994 and the chicken genome sequence was only reported in 2004. It is noteworthy that the identity of interferon-stimulated genes is far better described in mammals. Although there are increasing numbers of reports of chicken responses to virus infection, most of the regulated genes described are involved in the cell cycle, metabolic pathways and cellular structures required for virus replication. We have conducted a study aimed at describing the complement of potential anti-viral genes, identifying those induced by recombinant type I interferon in a common research and vaccine production substrate, namely primary chick embryo fibroblasts. The results are robust because 155 of the 193 genes induced were detected by at least two of the three transcriptomic technologies employed: Illumina RNA-seq, the Affymetrix 32K GeneChip Chicken Genome Array and the Chicken Gene 1.0 ST Array.