Phylogenetic analysis, genomic organization, and expression analysis of multi-copper oxidases in the ectomycorrhizal basidiomycete Laccaria bicolor
Phylogenetic analysis, genomic organization, and expression analysis of multi-copper oxidases in the ectomycorrhizal basidiomycete Laccaria bicolor
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DOI：
10.1111/j.1469-8137.2009.02774.x
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发表时间：
2009-01-01
期刊：
影响因子：
9.4
通讯作者：
Kuees, U.
中科院分区：
文献类型：
--
作者：
Courty, P. E.;Hoegger, P. J.;Kuees, U.
在森林土壤中，外生菌根真菌和腐养真菌的碳获取策略不同，但具有共同的编码多铜氧化酶（MCOs）的基因家族。这些酶参与多种土壤有机化合物的氧化。外生菌根真菌双色拉菌的MCO基因家族由11个基因组成，分为两个不同的亚家族，分别对应于严格意义上的漆酶（lcc）基因（lcc1至lcc9），与嗜铜的灰葡萄球菌漆酶基因和灰葡萄球菌不存在的氧化铁酶（lcc10和lcc11）具有高度的序列同源性。双色l中fe3样铁氧化酶基因lcc10和lcc11分别与编码铁渗透酶的ftr基因呈镜像串联排列。与C. cinerea不同，L. bicolor没有用于铁载体生物合成的sid1/sidA基因。利用全基因组表达阵列和定量逆转录聚合酶链反应（qRT-PCR）对转录本进行分析发现，在外生菌根（lcc3和lcc8）、子实体（lcc7）和琼脂培养基上生长的菌丝体（lcc9和lcc10）中，一些转录本非常丰富，表明这些MCOs具有特定的功能。MCO底物结合位点的氨基酸组成表明双色L. MCO与不同于腐养真菌的底物相互作用。新植物学家（2009）182:736-750 doi: 10.1111/j.1469-8137.2009.02774.x。
In forest soils, ectomycorrhizal and saprotrophic Agaricales differ in their strategies for carbon acquisition, but share common gene families encoding multi-copper oxidases (MCOs). These enzymes are involved in the oxidation of a variety of soil organic compounds.The MCO gene family of the ectomycorrhizal fungus Laccaria bicolor is composed of 11 genes divided into two distinct subfamilies corresponding to laccases (lcc) sensu stricto (lcc1 to lcc9), sharing a high sequence homology with the coprophilic Coprinopsis cinerea laccase genes, and to ferroxidases (lcc10 and lcc11) that are not present in C. cinerea. The fet3-like ferroxidase genes lcc10 and lcc11 in L. bicolor are each arranged in a mirrored tandem orientation with an ftr gene coding for an iron permease. Unlike C. cinerea, L. bicolor has no sid1/sidA gene for siderophore biosynthesis.Transcript profiling using whole-genome expression arrays and quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) revealed that some transcripts were very abundant in ectomycorrhizas (lcc3 and lcc8), in fruiting bodies (lcc7) or in the free-living mycelium grown on agar medium (lcc9 and lcc10), suggesting a specific function of these MCOs.The amino acid composition of the MCO substrate binding sites suggests that L. bicolor MCOs interact with substrates different from those of saprotrophic fungi.New Phytologist (2009) 182: 736-750 doi: 10.1111/j.1469-8137.2009.02774.x.