Changes in calbindin-D28k and parvalbumin expression in the superior olivary complex following unilateral cochlear ablation in neonatal rats
Changes in calbindin-D28k and parvalbumin expression in the superior olivary complex following unilateral cochlear ablation in neonatal rats
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DOI：
10.1080/00016480802455283
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发表时间：
2009-01-01
影响因子：
1.4
通讯作者：
Ito, Makoto
中科院分区：
文献类型：
--
作者：
Hatano, Miyako;Furukawa, Mitsuru;Ito, Makoto
结论单侧先天性耳聋伴耳蜗核体积减少引起对侧上级橄榄复合体（SOC）钙结合蛋白（CaBP）的变化。随着CN中神经元的损失和体积的减小，可能发生对侧SOC的输入减少，这导致这些核中CaBP的下调。本研究为聋儿听性脑干的神经化学研究提供了一些启示。Objective.在早期发育期间由耳蜗损伤产生的听力损失可导致成年人中枢听觉系统组织的持续变化。本研究的目的是探讨单侧耳蜗消融大鼠听觉脑干神经化学的变化。材料和方法。在早期发育过程中单侧耳蜗消融后，我们研究了两种CaBP，钙结合蛋白-D28 k（CB）和小清蛋白（PV）在SOC中的分布变化。成年后，CB和PV免疫反应性神经元显着减少，观察到对侧SOC，特别是在内侧核的梯形体（MNTB），虽然没有观察到神经元细胞死亡。还观察到同侧CN的体积减小。
Conclusion. Unilateral congenital deafness with a volume reduction in cochlear nucleus (CN) induced changes in the calcium-binding proteins (CaBPs) in the contralateral superior olivary complex (SOC) in the rat. With the loss of neurons and a volume reduction in the CN, a decrease in the input to the contralateral SOC may occur, which results in the down-regulation of CaBPs in these nuclei. This study may provide some implications regarding the neurochemistry in the auditory brainstem of deaf children. Objective. Hearing loss produced by cochlear damage during early development can result in persistent changes in the organization of the central auditory system in adults. The purpose of the present study was to investigate the neurochemical changes produced in the auditory brainstem of rats with unilateral cochlear ablation conducted before the onset of hearing. Materials and methods. Following unilateral cochlear ablation during early development, we examined the changes in the distribution of two CaBPs, calbindin-D28k (CB) and parvalbumin (PV), in the SOC. Results. Upon reaching adulthood, a marked decrease in CB- and PV-immunoreactive neurons was observed in the contralateral SOC, particularly in the medial nucleus of the trapezoid body (MNTB), although no neuronal cell death was observed. A volume reduction in the ipsilateral CN was also observed.