Rapid Detection of E. coli Bacteria Using Potassium-Sensitive FETs in CMOS
Rapid Detection of E. coli Bacteria Using Potassium-Sensitive FETs in CMOS
复制标题
使用 CMOS 中的钾敏感 FET 快速检测大肠杆菌
DOI：
10.1109/tbcas.2013.2276013
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发表时间：
2013
影响因子：
5.1
通讯作者：
K. Maxwell
中科院分区：
文献类型：
--
作者：
N. Nikkhoo;P. G. Gulak;K. Maxwell
提出了一种新的集成系统，可在10分钟内检测出活菌。它利用噬菌体作为生物检测元件的特异性，采用传统0.18 μm CMOS集成离子选择场效应晶体管（isfet）的灵敏度，并附加后处理pvc基钾敏感膜，提供快速，低成本的细菌检测平台。介绍了消除ISFET非理想性的实验方法和提高细菌传感器检测能力的数据处理技术。在两种不同的温度下，用四株带有两种噬菌体的大肠杆菌提供了三组实验结果。结合阳性和阴性控制实验的测量，成功地展示了传感器特异性和检测能力。
A novel integrated system for the detection of live bacteria in less than 10 minutes is presented. It utilizes the specificity of bacteriophages as biological detection elements with the sensitivity of integrated ion-selective field-effect transistors (ISFETs) implemented in conventional 0.18 μm CMOS with additional post-processes PVC-based potassium-sensitive membrane to provide a rapid, low-cost bacteria detection platform. Experimental methods to cancel ISFET non-idealities as well as data processing techniques to enhance detection capability of the bacteria sensor are demonstrated. Three groups of experimental results are provided using four strains of E. coli with two bacteriophages at two different temperatures. Measurements incorporating positive and negative control experiments are presented that successfully exhibit sensor specificity as well detection capability.