Hybrid biocomposite with a tunable antibacterial activity and bioactivity based on RF magnetron sputter deposited coating and silver nanoparticles
Hybrid biocomposite with a tunable antibacterial activity and bioactivity based on RF magnetron sputter deposited coating and silver nanoparticles
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DOI：
10.1016/j.apsusc.2014.12.153
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发表时间：
2015-02-28
影响因子：
6.7
通讯作者：
Epple, M.
中科院分区：
文献类型：
--
作者：
Ivanova, A. A.;Surmenev, R. A.;Epple, M.
在这项工作中，我们描述了用于制备基于羟基磷灰石（HA）涂层和银纳米粒子（AgNPs）的多功能生物复合材料的制造技术。采用湿法化学还原法制备AgNPs，采用滴干法在Ti衬底上沉积AgNPs，然后采用射频磁控溅射沉积法制备磷酸钙（Cap）涂层。带负电荷的银纳米粒子（zeta电位-21 mV）呈球形，金属芯直径为50 +/- 20 nm。HA涂层作为致密的纳米晶膜沉积在AgNPs表面上。射频磁控溅射沉积HA膜在AgNPs层上不影响衬底表面AgNPs的初始含量，也不影响NPs的尺寸和形状。使用后向散射模式拍摄的SEM横截面图像显示，在CaP层下有一层均匀的AgNPs。涂层的衍射图显示了结晶HA和银的反射。测定了生物复合材料在磷酸盐和醋酸盐缓冲液中浸泡7天后释放的银离子浓度。结果表明，磷酸盐缓冲液和乙酸缓冲液溶液中银的含量分别为0.27 +/- 0.02 μ g mL（-1）和0.54 +/- 0.02 μ g mL(-1)，与文献中已知的银离子的最小抑制浓度范围相吻合。因此，本工作建立了一条利用嵌入AgNPs制备生物相容性层以实现局部抗菌效果的新途径。(C) 2014 Elsevier B.V.版权所有
In this work, we describe fabrication techniques used to prepare a multifunctional biocomposite based on a hydroxyapatite (HA) coating and silver nanoparticles (AgNPs). AgNPs synthesized by a wet chemical reduction method were deposited on Ti substrates using a dripping/drying method followed by deposition of calcium phosphate (Cap) coating via radio-frequency (RF) magnetron sputter-deposition. The negatively charged silver nanoparticles (zeta potential -21 mV) have a spherical shape with a metallic core diameter of 50 +/- 20 nm. The HA coating was deposited as a dense nanocrystalline film over a surface of AgNPs. The RF-magnetron sputter deposition of HA films on the AgNPs layer did not affect the initial content of AgNPs on the substrate surface as well as NPs size and shape. SEM cross-sectional images taken using the backscattering mode revealed a homogeneous layer of AgNPs under the CaP layer. The diffraction patterns from the coatings revealed reflexes of crystalline HA and silver. The concentration of Ag ions released from the biocomposites after 7 days of immersion in phosphate and acetate buffers was estimated. The obtained results revealed that the amount of silver in the solutions was 0.27 +/- 0.02 mu g mL(-1) and 0.54 +/- 0.02 mu g mL(-1) for the phosphate and acetate buffers, respectively, which corresponded well with the minimum inhibitory concentration range known for silver ions in literature. Thus, this work establishes a new route to prepare a biocompatible layer using embedded AgNPs to achieve a local antibacterial effect. (C) 2014 Elsevier B.V. All rights reserved.