The lipid droplet is an important organelle for hepatitis C virus production
The lipid droplet is an important organelle for hepatitis C virus production
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DOI：
10.1038/ncb1631
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发表时间：
2007-09-01
影响因子：
21.3
通讯作者：
Shimotohno, Kunitada
中科院分区：
文献类型：
--
作者：
Miyanari, Yusuke;Atsuzawa, Kimie;Shimotohno, Kunitada
脂滴（LD）是一种用于储存中性脂质的细胞器。它在细胞质中动态移动，与其他细胞器相互作用，包括内质网（ER）（1-3）。这些相互作用被认为有助于脂质和蛋白质向其他细胞器的运输。丙型肝炎病毒（HCV）是慢性肝病的病原体（4）。HCV衣壳蛋白（核心）与LD 5结合，包膜蛋白E1和E2位于ER腔中（6），病毒复制酶被认为定位于ER衍生的膜上。然而，人们对HCV颗粒如何以及在何处组装知之甚少。在这里，我们表明，LD参与生产的传染性病毒颗粒。我们证明，核心招聘非结构（NS）蛋白和复制复合物的LD相关的膜，这种招聘是至关重要的生产传染性病毒。此外，在LD附近观察到病毒颗粒，表明病毒组装的一些步骤发生在LD周围。这项研究揭示了一个新的功能，LD在组装感染性HCV，并提供了一个新的视角，病毒如何篡夺细胞功能。
The lipid droplet (LD) is an organelle that is used for the storage of neutral lipids. It dynamically moves through the cytoplasm, interacting with other organelles, including the endoplasmic reticulum ( ER)(1-3). These interactions are thought to facilitate the transport of lipids and proteins to other organelles. The hepatitis C virus (HCV) is a causative agent of chronic liver diseases(4). HCV capsid protein ( Core) associates with the LD5, envelope proteins E1 and E2 reside in the ER lumen(6), and the viral replicase is assumed to localize on ER- derived membranes. How and where HCV particles are assembled, however, is poorly understood. Here, we show that the LD is involved in the production of infectious virus particles. We demonstrate that Core recruits nonstructural (NS) proteins and replication complexes to LD-associated membranes, and that this recruitment is critical for producing infectious viruses. Furthermore, virus particles were observed in close proximity to LDs, indicating that some steps of virus assembly take place around LDs. This study reveals a novel function of LDs in the assembly of infectious HCV and provides a new perspective on how viruses usurp cellular functions.