Rat histamine N-methyltransferase. Quantification, tissue distribution, purification, and immunologic properties.
Rat histamine N-methyltransferase. Quantification, tissue distribution, purification, and immunologic properties.
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大鼠组胺 N-甲基转移酶。
DOI：
10.1016/s0021-9258(17)44159-7
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
D. Henry
中科院分区：
文献类型：
--
作者：
R. Bowsher;K. Verburg;D. Henry
组胺N-甲基转移酶是哺乳动物组织中使组胺失活的主要酶。开发了一种灵敏和特异的组胺N-甲基转移酶测定方法，可在25种大鼠组织中定量测定该活性。此外，将肾组胺N-甲基转移酶纯化至电泳均一性。纯化方案中的关键步骤是亲和柱程序，其涉及用1 mM组胺从DEAE-Sephacel特异性洗脱组胺N-甲基转移酶。纯化的酶具有33，400的分子量和窄的pH依赖性，最适pH为8.0-8.25。肾组织胺N-甲基转移酶的特异性抗体也完全免疫沉淀脑组织胺N-甲基转移酶，因此表明神经元和非神经元组织胺N-甲基转移酶具有共同的抗原决定簇。
Histamine N-methyltransferase is a major enzyme for inactivating histamine in mammalian tissues. Development of a sensitive and specific assay for histamine N-methyltransferase has permitted quantification of this activity in 25 rat tissues. Additionally, renal histamine N-methyltransferase was purified to electrophoretic homogeneity. The pivotal step in the purification scheme was an affinity column procedure which involved the specific elution of histamine N-methyltransferase from DEAE-Sephacel by 1 mM histamine. The purified enzyme has a molecular weight of 33,400 and a narrow pH dependency with an optimum at 8.0-8.25. Specific antibody produced to renal histamine N-methyltransferase also immunoprecipitated the brain enzyme completely, therefore indicating neuronal and non-neuronal histamine N-methyltransferase share common antigenic determinants.