Transplantation of preserved human amniotic membrane for bladder augmentation in rats
Transplantation of preserved human amniotic membrane for bladder augmentation in rats
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DOI：
10.1089/ten.2006.0170
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发表时间：
2007-03-01
影响因子：
--
通讯作者：
Nishizawa, Osamu
中科院分区：
文献类型：
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作者：
Iijima, Kazuyoshi;Igawa, Yasuhiko;Nishizawa, Osamu
虽然胃肠段已被广泛用于膀胱重建，但由于可能的并发症，它们并不理想。因此，仍在继续寻找另一种增强材料。在这里，我们使用人羊膜（hAM）对大鼠进行膀胱增强。形态学上，ham增强膀胱在术后3个月内显示尿路上皮、逼尿肌平滑肌和神经纤维再生。在膀胱条的功能评估中，我们比较了ham增强膀胱和小肠粘膜下层（SIS）增强膀胱。例如，在术后6个月，高钾、氯乙醇和电场刺激下，对照膀胱组的收缩大小（占反应的百分比）分别为：hAM 22% vs SIS 15%， hAM 15% vs SIS 7%, hAM 5.3% vs SIS 1.3% （hAM vs SIS无显著差异）。hAM和sis增强膀胱均显示出足够的容量和顺应性。目前的结果表明，对于膀胱增大，hAM可以用作支架，并且在这方面与SIS相当。火腿比SIS更容易获得，而且几乎不需要准备，而且它的使用几乎不会引发伦理问题。因此，hAM可能是泌尿系统重建的一种新的治疗选择。
Although gastrointestinal segments have been widely used for bladder reconstruction, they are not ideal because of the possible complications. Searches have therefore continued for an alternative material for augmentation. Here, we performed bladder augmentation in rats using human amniotic membrane (hAM). Morphologically, the hAM-augmented bladder revealed regeneration of urothelium, detrusor smooth muscle, and nerve fibers within 3 months post-operatively. In our functional evaluation of bladder strips, we compared hAM-augmented bladders with bladders augmented using small intestinal submucosa (SIS). For example, at 6 months post-operatively, contractions of the following size (as a percentage of the responses in the control-bladder group) were obtained in response to high potassium, carbachol, and electrical field stimulation, respectively: hAM 22% vs SIS 15%, hAM 15% vs SIS 7%, hAM 5.3% vs SIS 1.3% (no significant differences, hAM vs SIS). Both hAM- and SIS-augmented bladders displayed adequate capacity and compliance. The present results indicate that, for bladder augmentation, hAM can be used as a scaffold and is comparable in this respect with SIS. hAM can be more easily obtained than SIS and requires little preparation, and its use raises few ethical questions. Hence, hAM may represent a new therapeutic alternative for urological reconstructions.