Immune and clinical parameters associated with Leishmania infantum infection in the golden hamster model
Immune and clinical parameters associated with Leishmania infantum infection in the golden hamster model
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DOI：
10.1016/s0165-2427(00)00221-x
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发表时间：
2000-10-31
影响因子：
1.8
通讯作者：
Alonso, C
中科院分区：
文献类型：
--
作者：
Requena, JM;Soto, M;Alonso, C
对于嗜内脏利什曼原虫菌株的实验性感染，尚未确定合适的动物模型。在目前的工作中，我们重新评估了使用金仓鼠（Mesocricetus auratus）作为感染婴儿利什曼原虫的实验模型。各组仓鼠接受心内途径攻击，剂量范围为 10(3) 至 10(5) 感染性前鞭毛体，并对动物进行 1 年随访期监测。通过利什曼病的临床症状、肝脏和脾脏中的寄生虫负荷、对利什曼原虫抗原的体液反应以及肾脏中的抗体水平来评估感染的结果。使用粗抗原（通过 ELISA 和蛋白质印迹）或几种重组利什曼原虫抗原（Hsp70、Hsp83、LiP2a、LiP2b、H2A、H3 和 KMP-11）分析体液反应。通过对所有这些参数的分析，我们确定了三类动物的存在：有症状或易感、寡症状和耐药。鉴于仓鼠、狗和人类利什曼原虫感染结果之间存在相似性，我们相信我们的数据表明仓鼠是研究内脏利什曼病和疫苗开发设计的绝佳实验模型。 (C) 2000 Elsevier Science B.V. 保留所有权利。
For experimental infections with viscerotropic strains of Leishmania, a suitable animal model is not yet defined. In the present work, we have reappraised the use of golden hamster (Mesocricetus auratus) as an experimental model for infection with Leishmania infantum. Groups of hamsters were challenged by the intracardial route with doses ranging from 10(3) to 10(5) infectious promastigotes and the animals were monitored for 1-year follow-up period. The outcome of the infection was assessed by clinical symptoms of leishmaniasis, parasite loads in both liver and spleen, humoral response to Leishmania antigens and antibody levels in kidneys. The humoral response was analysed using either crude antigens (by ELISA and Western blotting) or several recombinant Leishmania antigens (Hsp70, Hsp83, LiP2a, LiP2b, H2A, H3 and KMP-11). From the analysis of all these parameters, we established the existence of three groups of animals: symptomatic or susceptible, oligosymptomatic, and resistant. Given the parallelism existing between the outcomes of Leishmania-infection in hamsters, dogs and humans, we believe that our data illustrate that the hamster is an excellent experimental model to study visceral leishmaniasis and for the design of vaccine development. (C) 2000 Elsevier Science B.V. All rights reserved.