Overexpression of SCC-S2 correlates with lymph node metastasis and poor prognosis in patients with non-small-cell lung cancer
Overexpression of SCC-S2 correlates with lymph node metastasis and poor prognosis in patients with non-small-cell lung cancer
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SCC-S2过表达与非小细胞肺癌患者淋巴结转移和不良预后相关
DOI：
10.1111/j.1349-7006.2010.01557.x
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发表时间：
2010-06-01
期刊：
影响因子：
5.7
通讯作者：
Wang, En-Hua
中科院分区：
文献类型：
--
作者：
Dong, Qian-Ze;Zhao, Yue;Wang, En-Hua
本研究旨在探讨SCC-S2在非小细胞肺癌（NSCLC）患者中的表达模式及其临床病理意义。采用实时RT-PCR、western blot和免疫组化检测SCC-S2在非小细胞肺癌组织及邻近非癌肺组织中的表达谱。在检查的25个肺癌组织中，18个（72%）的SCC-S2 mRNA水平高于相应的正常组织。肺癌组织中SCC-S2蛋白水平较邻近正常组织上调。采用免疫组化染色法检测93例NSCLC组织中SCC-S2的表达水平。SCC-S2在93例肺癌患者中有71例（76.3%）过表达，并与淋巴结转移（P = 0.0181）、P - tnm分期（P = 0.0042）、Ki-67表达（P = 0.0028）和生存率差（P = 0.012）相关。此外，通过小干扰RNA减少SCC-S2表达可抑制肺细胞系的生长和侵袭。这些结果表明，SCC-S2在非小细胞肺癌中起着重要的作用，可能是一个有用的治疗靶点。（癌症科学2010）。
The objective of the current study was to investigate the expression pattern and clinicopathological significance of SCC-S2 in patients with non-small-cell lung cancer (NSCLC). The expression profile of SCC-S2 in NSCLC tissues and adjacent noncancerous lung tissues was detected by real-time RT-PCR, western blot analysis, and immunohistochemistry. In 25 lung cancer tissues examined, 18 (72%) of them exhibited stronger levels of SCC-S2 mRNA compared with their corresponding normal tissues. SCC-S2 protein level was up-regulated in cancerous lung tissues compared to adjacent normal tissue. Moreover, the expression level of SCC-S2 in 93 archived NSCLC tissues was measured by immunohistochemical staining. SCC-S2 was found to be overexpressed in 71 of 93 (76.3%) human lung cancer samples and correlated with lymph node metastasis (P = 0.0181), p-TNM stage (P = 0.0042), Ki-67 expression (P = 0.0028), and poor survival (P = 0.012). In addition, depleting SCC-S2 expression by small-interfering RNA inhibited growth and invasion in lung cell lines. These results indicate that SCC-S2 plays an important role in NSCLC and might be a useful therapeutic target of NSCLC. (Cancer Sci 2010).