Systemic transplantation of allogenic fetal membrane-derived mesenchymal stem cells suppresses Th1 and Th17 T cell responses in experimental autoimmune myocarditis
Systemic transplantation of allogenic fetal membrane-derived mesenchymal stem cells suppresses Th1 and Th17 T cell responses in experimental autoimmune myocarditis
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DOI：
10.1016/j.yjmcc.2012.06.020
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发表时间：
2012-09-01
影响因子：
5
通讯作者：
Ikeda, Tomoaki
中科院分区：
文献类型：
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作者：
Ohshima, Makiko;Yamahara, Kenichi;Ikeda, Tomoaki
我们已经报道，自体骨髓或同种异体胎膜（FM）衍生的间充质干细胞（MSC）的全身给药同样减轻了实验性自身免疫性心肌炎（EAM）大鼠的心肌损伤。由于大鼠EAM是一种辅助性T细胞（Th）介导的自身免疫性疾病，最近的证据表明，自体和同种异体间充质干细胞发挥免疫抑制作用的Th细胞活性，我们专注于Th细胞分化同种异体FM-MSC管理EAM大鼠。通过注射猪心肌肌球蛋白在刘易斯大鼠中诱导EAM（第0天）。在第7、10或14天（分别为MSCd 7、MSCd 10或MSCd 14组）静脉内注射（5 x 10个细胞/大鼠）从主要组织相容性复合物不匹配的ACI大鼠中获得的同种异体FM-MSC。在第21天，超声心动图证实未治疗EAM组射血分数降低（63 +/- 2%）在MSCd 10和MSCd 14组显著改善（分别为74 +/- 1和75 +/-2%，P
We have reported that systemic administration of autologous bone marrow or allogenic fetal membrane (FM)-derived mesenchymal stem cells (MSCs) similarly attenuated myocardial injury in rats with experimental autoimmune myocarditis (EAM). Since rat EAM is a T-helper (Th) cell-mediated autoimmune disease, and recent evidence has indicated that both autologous and allogenic MSCs exert an immunosuppressive effect on Th cell activity, we focused on Th cell differentiation in allogenic FM-MSC administered EAM rats. EAM was induced in Lewis rats by injecting porcine cardiac myosin (day 0). Allogenic FM-MSCs, obtained from major histocompatibility complex mismatched ACI rats, were intravenously injected (5 x 10 cells/rat) on days 7, 10, or 14 (MSCd7, MSCd10, or MSCd14 groups, respectively). At day 21, echocardiography confirmed that reduced ejection fraction in the untreated EAM group (63 +/- 2%) was significantly improved in the MSCd10 and MSCd14 groups (74 +/- 1 and 75 +/- 2%, respectively, P