MicroRNA expression profiles in human cancer cells after ionizing radiation.
MicroRNA expression profiles in human cancer cells after ionizing radiation.
复制标题
DOI：
10.1186/1748-717x-6-29
复制
发表时间：
2011-03-31
期刊：
影响因子：
--
通讯作者：
Belka C
中科院分区：
文献类型：
--
作者：
Niemoeller OM;Niyazi M;Corradini S;Zehentmayr F;Li M;Lauber K;Belka C
microRNA是中枢细胞过程的调节因子，与人类癌症的发病机制和预后有关。MicroRNA还调节对抗癌治疗的反应。在放射肿瘤学的背景下，发现microRNA在照射后调节细胞死亡和增殖。然而，到目前为止，还没有全面分析辐射对microRNA表达谱的影响。本研究的目的是提供一个广泛的屏幕上的变化，在不同的恶性肿瘤细胞系照射后的microRNA表达。在用2.0戈伊的临床相关剂量照射后，在六种恶性细胞系中分析1100种微RNA（桑格miRBase发布版本14.0）。使用"Geniom Biochip MPEA homo sapiens"将照射后6小时的微RNA水平与未照射细胞中的微RNA水平进行比较。分层聚类分析揭示了一种模式，它显着（p = 0.014）区分辐射从非辐射细胞。已知参与细胞过程如细胞凋亡、增殖、侵袭、局部免疫应答和辐射抗性（例如，G. miR-1285、miR-24 - 1、miR-151 - 5p、let-7i）在照射后显示2 - 3倍的变化。此外，还发现了一些以前不知道的辐射响应性microRNA。电离辐射诱导3种胶质瘤和3种鳞状细胞癌细胞株microRNA表达谱发生显著变化。这些变化的功能相关性未得到解决，但应通过未来的工作进行分析，特别是关注临床相关终点，如辐射诱导的细胞死亡、增殖、迁移和转移。
MicroRNAs are regulators of central cellular processes and are implicated in the pathogenesis and prognosis of human cancers. MicroRNAs also modulate responses to anti-cancer therapy. In the context of radiation oncology microRNAs were found to modulate cell death and proliferation after irradiation. However, changes in microRNA expression profiles in response to irradiation have not been comprehensively analyzed so far. The present study's intend is to present a broad screen of changes in microRNA expression following irradiation of different malignant cell lines. 1100 microRNAs (Sanger miRBase release version 14.0) were analyzed in six malignant cell lines following irradiation with clinically relevant doses of 2.0 Gy. MicroRNA levels 6 hours after irradiation were compared to microRNA levels in non-irradiated cells using the "Geniom Biochip MPEA homo sapiens". Hierarchical clustering analysis revealed a pattern, which significantly (p = 0.014) discerned irradiated from non-irradiated cells. The expression levels of a number of microRNAs known to be involved in the regulation of cellular processes like apoptosis, proliferation, invasion, local immune response and radioresistance (e. g. miR-1285, miR-24-1, miR-151-5p, let-7i) displayed 2 - 3-fold changes after irradiation. Moreover, several microRNAs previously not known to be radiation-responsive were discovered. Ionizing radiation induced significant changes in microRNA expression profiles in 3 glioma and 3 squamous cell carcinoma cell lines. The functional relevance of these changes is not addressed but should by analyzed by future work especially focusing on clinically relevant endpoints like radiation induced cell death, proliferation, migration and metastasis.