Interstitial cells in deep muscular plexus of canine small intestine may be specialized smooth muscle cells.
Interstitial cells in deep muscular plexus of canine small intestine may be specialized smooth muscle cells.
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犬小肠深层肌丛中的间质细胞可能是特化的平滑肌细胞。
DOI：
10.1152/ajpgi.1993.265.4.g638
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Sanders,KM
中科院分区：
文献类型：
--
作者：
Torihashi,S;Kobayashi,S;Gerthoffer,WT;Sanders,KM
用透射电子显微镜、免疫细胞化学和扫描电子显微镜对犬小肠内、外环状肌层间细胞的精细结构和性质进行了研究。观察到一种由肠上皮细胞、血管周围的成纤维细胞、巨噬细胞以及以前被认为是Cajal间质细胞的细小的分支细胞支持的神经丛(深肌丛)。深肌丛间质细胞(IC-DMP)富含线粒体、致密小体和小凹，与神经纤维关系密切。IC-DMP基底板不完整。这些细胞也有许多相互连接的缝隙连接，它们还与周围的外环肌层的平滑肌细胞形成缝隙连接。IC-DMP富含肌丝，主要为肌动蛋白细丝，但抗肌球蛋白轻链抗体鉴定的肌球蛋白粗丝也很明显。IC-DMP和环状平滑肌细胞均表达抗平滑肌肌动蛋白抗血清，但两者的中间丝蛋白不同：IC-DMP细胞呈波形蛋白免疫阳性，环状平滑肌细胞呈结蛋白免疫反应。扫描电子显微镜显示IC-DMP胞体薄而扁平，有大量的分支突起。这些细胞与神经纤维和环状平滑肌细胞密切接触。IC-DMP细胞含有肌球蛋白粗丝，且抗平滑肌肌动蛋白免疫阳性，提示IC-DMP细胞可能更适合被归类为一种平滑肌细胞。
The fine structures and properties of cells between the inner and outer circular muscle layers in the canine small intestine were studied by transmission electron microscopy (TEM), immunocytochemistry, and scanning electron microscopy (SEM). A nerve plexus (deep muscular plexus) supported by enteroglial cells, fibroblasts around blood vessels, macrophages, and thin and branched cells previously identified as interstitial cells of Cajal was observed. The interstitial cells of the deep muscular plexus (IC-DMP) were rich in mitochondria, dense bodies, and caveolae, and they were closely associated with nerve fibers. The IC-DMP had incomplete basal laminae. These cells also had numerous interconnecting gap junctions, and they also formed gap junctions with the surrounding smooth muscle cells of the outer circular muscle layer. IC-DMP were rich in myofilaments, which were primarily actin thin filaments, but myosin thick filaments, identified with anti-myosin light-chain antibodies, were also apparent. IC-DMP and circular smooth muscle cells both expressed immunoreactivity to anti-smooth muscle actin antisera, but these two types of cells differed in their intermediate filament proteins: IC-DMP featured vimentin immunopositive filaments, and circular smooth muscle cells featured desmin immunoreactivity. SEM showed that IC-DMP had thin and flat cell bodies with numerous branching processes. These cells came into close contact with nerve fibers and circular smooth muscle cells. The findings that IC-DMP cells contained myosin thick filaments and were immunopositive for anti-smooth muscle actin suggest that they may be more properly categorized as a type of smooth muscle cell.