Coronary flow rate dependent enzyme release rate from normoxic and anoxic rat hearts in vitro.
Coronary flow rate dependent enzyme release rate from normoxic and anoxic rat hearts in vitro.
复制标题
体外常氧和缺氧大鼠心脏的冠状动脉流速依赖性酶释放率。
DOI：
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发表时间：
1984
影响因子：
10.8
通讯作者：
P. Oemrawsingh
中科院分区：
文献类型：
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作者：
A. van der Laarse;P. V. Van dijkman;J. Altona;A. Zoet;P. Oemrawsingh
为探讨冠脉流量(CFR)与离体大鼠常氧和缺氧心脏酶释放速率的关系，我们测定了3h试验期内心脏释放的乳酸脱氢酶(LDH)的总活性和此期间的平均CFR。试验期包括在氧气和丙酮酸钠存在下(欠)灌流(常氧性缺血)或在无氧和代谢底物(缺氧性缺血)的情况下(欠)灌流。在0~17mlX min-1范围内，缺氧性缺血组和CFR在0~7mlXmin-1范围内的常氧性缺血期LDH活性与平均CFR呈正相关。当CFR>7ml×min-1时，常氧缺血3h释放的LDH活性与CFR呈负相关。平均CFR与3h末冠脉阻力呈负相关。在常氧缺血的心脏中，冠脉阻力与心肌未灌注率呈正相关。这项研究表明，低CFR会导致心肌灌注不均匀，从而抑制酶从心肌细胞渗漏到灌流液中。
To study the relation between coronary flow rate (CFR) and the rate of enzyme release from isolated perfused rat hearts which were either normoxic or anoxic, we measured the total activity of lactate dehydrogenase (LDH) released from the heart during a 3 h test period and mean CFR during that period. The test period consisted of (under)perfusion in the presence of oxygen and sodium pyruvate (normoxic ischaemia) or (under)perfusion in the absence of oxygen and metabolic substrates (anoxic ischaemia). Activity of LDH released during the 3 h test period correlated positively with mean CFR during anoxic ischaemia for the whole range of CFR from 0 to 17 ml X min-1, and during normoxic ischaemia when CFR was in the range 0 to 7 ml X min-1. At a CFR higher than 7 ml X min-1, the activity of LDH released during 3 h of normoxic ischaemia correlated negatively with CFR. Mean CFR was negatively correlated with coronary vascular resistance at the end of the 3 h period of perfusion. In the hearts subjected to normoxic ischaemia, coronary vascular resistance correlated positively with the percentage of myocardium unperfused. This study shows that a low CFR leads to unhomogeneous myocardial perfusion, which inhibits leakage of enzymes from myocytes into the perfusate.