Vasculitis and anaphylactoid shock in mice induced by the polysaccharide fraction secreted into culture supernatants by the fungus Candida metapsilosis
Vasculitis and anaphylactoid shock in mice induced by the polysaccharide fraction secreted into culture supernatants by the fungus Candida metapsilosis
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真菌化生念珠菌分泌到培养上清液中的多糖组分诱导小鼠血管炎和过敏性休克
DOI：
10.1111/j.1348-0421.2011.00326.x
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发表时间：
2011
影响因子：
2.6
通讯作者：
Naohito Ohno
中科院分区：
文献类型：
--
作者：
Rui Tada;Yusuke Takano;Hisashi Murakami;Ken-ichi Ishibashi;Noriko Nagi-Miura;Yoshiyuki Adachi;Naohito Ohno
检查了使用完全合成培养基制备的间质假丝酵母水溶性组分（CMWS）的生物学效应，以确定CMWS是否诱导小鼠中与川崎和过敏性休克相似的血管炎。结果发现，腹腔注射CMWS可诱导小鼠冠状动脉炎，静脉注射可诱导小鼠急性过敏性休克，与白色念珠菌水溶性部分（CAWS）诱导的动脉炎和过敏性休克相似。然后通过进行抗血清反应性试验和核磁共振光谱分析多糖级分的甘露聚糖结构。甘露聚糖结构进行了研究，因为本作者最近发现，多糖馏分内的甘露聚糖部分可能是负责这些致病活动。结构分析表明，CMWS中的甘露聚糖结构表达α-甘露聚糖残基，但不表达β-甘露聚糖。此外，CMWS的甘露聚糖结构与CAWS非常相似。结果表明，C.以甘露聚糖为主要成分的代谢产物可引起冠状动脉炎和急性休克，甘露聚糖结构可能是其致病机制之一。
The biological effects ofCandida metapsilosiswater‐soluble fraction (CMWS), prepared using a completely synthesized medium, were examined to determine whether CMWS induces vasculitis similar to that seen in Kawasaki disease, and anaphylactoid shock, in mice. It was found that intraperitoneal injection of CMWS induces coronary arteritis and i.v. injection induces acute anaphylactoid shock in mice, similar toCandida albicanswater‐soluble fraction (CAWS)‐induced arteritis and anaphylactoid shock. The mannan structure of the polysaccharide fraction was then analyzed by performing antiserum reactivity tests and nuclear magnetic resonance spectroscopy. The mannan structure was investigated because the present authors have recently found that the mannan moiety within the polysaccharide fraction might be responsible for these pathogenic activities. The structural analysis showed that the mannan structure within CMWS expresses α‐mannan residues, but not β‐mannan. In addition, the mannan structure of CMWS is quite similar to that of CAWS. The present findings indicate that the polysaccharide fraction fromC. metapsilosis, which is mainly composed of mannan, contributes to coronary arteritis and acute shock, and that the mannan structure could be responsible for this pathogenicity.