Identification of QTL influencing seed oil content, fatty acid profile and days to flowering in Brassica napus L.
Identification of QTL influencing seed oil content, fatty acid profile and days to flowering in Brassica napus L.
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DOI：
10.1007/s10681-015-1565-2
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发表时间：
2016-01-01
期刊：
影响因子：
1.9
通讯作者：
Duncan, Robert W.
中科院分区：
文献类型：
--
作者：
Javed, Nasir;Geng, Jianfeng;Duncan, Robert W.
本研究旨在鉴定控制甘蓝型油菜含油量、脂肪酸组成和开花期的数量性状基因座（QTL）。本研究利用来自波罗9 Topas杂交的加倍单倍体作图群体进行。在四种环境中对种群进行表型分析。QTL分析的复合区间法进行了先前可用的遗传图谱，主要由简单的重复序列标记，平均遗传距离为3.7 cM。将标记组装并锚定到19条染色体上，图谱覆盖率为2244.1 cM。共检测到14个与油分含量相关的QTL，131个与6种脂肪酸相关的QTL，14个与开花时间相关的QTL。一个含有Topas等位基因的QTL qOIL-A10 a可解释26.99%的含油量变异。此外，含油量的超亲分离也超过了亲本表型（31.5- 55.5%）. C3上的两个基因组区域，在147.83和154.55 cM与QTL的所有六种脂肪酸的研究。我们假设C3上的这个基因组区域调节脂肪酸之间的相关性，并且对该区域的进一步研究可以提供对决定B中总种子油含量的基因的深入了解。油菜。在已知的芸苔属春化基因附近检测到一个含有Polo等位基因的早花QTL qFLRa-A2 c，该QTL可解释43.22%的性状变异。性状间的表型相关性和34个基因组区域上不同QTL的配置表明，所研究的性状之间存在遗传依赖性。
The objective of this study was to identify quantitative trait loci (QTL) controlling oil content, fatty acid profile and flowering time in Brassica napus L. This research was conducted using a doubled haploid mapping population derived from a cross of Polo 9 Topas. The population was phenotyped in four environments. The composite interval method of QTL analysis was performed with a previously available genetic map that consisted of mainly simple sequence repeat markers with an average genetic distance of 3.7 cM. The markers were assembled and anchored to 19 chromosomes with a map coverage of 2244.1 cM. Fourteen QTL were identified for oil content, 131 QTL were found to be associated with six fatty acids and 14 QTL were associated with flowering time. A QTL, qOIL-A10a with a positive Topas-allele explained 26.99 % of the variation in oil content. Additionally, transgressive segregation for oil content was observed beyond the parental phenotypes (31.5-55.5 %). Two genomic regions on C3, at 147.83 and 154.55 cM were associated with QTL for all six fatty acids studied. We hypothesize this genomic region on C3 modulates the correlations between fatty acids and further investigation of this region could provide insight into the genes determining total seed oil content in B. napus. An early flowering QTL, qFLRa-A2c containing a Polo-allele was detected in the vicinity of a known Brassica vernalization gene that explained 43.22 % of the trait variation. The phenotypic correlation between traits and collocation of different QTL on thirty-four genomic regions suggests that the traits studied have genetic dependencies on each other.