Multiple high-throughput analyses monitor the response of E-coli to perturbations
Multiple high-throughput analyses monitor the response of E-coli to perturbations
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DOI：
10.1126/science.1132067
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发表时间：
2007-04-27
期刊：
影响因子：
56.9
通讯作者：
Tomita, Masaru
中科院分区：
文献类型：
--
作者：
Ishii, Nobuyoshi;Nakahigashi, Kenji;Tomita, Masaru
对生物信息的多层次细胞成分的分析可以提供有价值的功能洞察。我们进行了多次高通量测量，以研究大肠杆菌细胞对遗传和环境扰动的反应。对代谢酶基因干扰物的分析显示，大多数干扰物的信使RNA和蛋白质发生了出人意料的微小变化。总体而言，代谢物水平也是稳定的，反映出代谢网络中的通量发生了改变。相比之下，大肠杆菌主动调节酶水平，以保持稳定的代谢状态，以响应生长速度的变化。因此，大肠杆菌似乎使用了互补的策略，导致了一个强大的新陈代谢网络，以抵御干扰。
Analysis of cellular components at multiple levels of biological information can provide valuable functional insights. We performed multiple high-throughput measurements to study the response of Escherichia coli cells to genetic and environmental perturbations. Analysis of metabolic enzyme gene disruptants revealed unexpectedly small changes in messenger RNA and proteins for most disruptants. Overall, metabolite levels were also stable, reflecting the rerouting of fluxes in the metabolic network. In contrast, E. coli actively regulated enzyme levels to maintain a stable metabolic state in response to changes in growth rate. E. coli thus seems to use complementary strategies that result in a metabolic network robust against perturbations.