THE INVIVO AND INVITRO GLOMERULAR DEPOSITION OF ISOLATED ANTI-DOUBLE-STRANDED-DNA ANTIBODIES IN NZB/W MICE
THE INVIVO AND INVITRO GLOMERULAR DEPOSITION OF ISOLATED ANTI-DOUBLE-STRANDED-DNA ANTIBODIES IN NZB/W MICE
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DOI：
10.1016/0090-1229(84)90061-8
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
HARBECK, RJ
中科院分区：
文献类型：
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作者：
DANG, H;HARBECK, RJ
采用羟基磷灰石双链DNA（ds-DNA）亲和层析柱，从老龄NZB/W小鼠血清中分离抗ds-DNA抗体。将这些抗体放射性标记并静脉注射到不同年龄的NZB/W小鼠中。NZB/W小鼠4个月时放射性标记抗体显著沉积。年龄和年龄。虽然注射的抗体在其等电点（pI）中显示出异质性，但与肾脏结合的抗体仅限于碱性更强的pI范围。从不同年龄的NZB/W小鼠中制备肾小球培养物，并与具有限制性pI的放射性标记抗ds-DNA抗体孵育。pI在7-9之间的抗体在体外与从9个月获得的肾小球结合。老年NZB/W小鼠，但不适用于2 mo.老老鼠pI在5-7之间的抗体不能与从任一年龄组获得的肾小球结合。游离抗体和结合到肾小球的抗体之间的交换在体外发生。研究结果表明存在抗ds-DNA抗体的亚群，其优先结合含有免疫复合物的肾小球。
Employing a hydroxyapatite double-stranded (ds) DNA column for affinity chromatography, anti-ds-DNA antibodies were isolated from the sera of aging NZB/W mice. These antibodies were radiolabeled and injected i.v. into NZB/W mice at various ages. Significant deposition of radiolabeled antibodies occurred in NZB/W mice 4 mo. of age and older. While the injected antibodies displayed heterogeneity in their isoelectric points (pI), those antibodies which bound to the kidney were restricted to a more alkaline pI range. Glomerular cultures were prepared from NZB/W mice at various ages and incubated with radiolabeled anti-ds-DNA antibodies with restricted pI. Antibodies having pI between 7-9 bound in vitro to glomeruli obtained from 9 mo. old NZB/W mice but not to glomeruli obtained from 2 mo. old mice. Antibodies with pI between 5-7 were unable to bind to glomeruli obtained from either age group. An exchange between free antibodies and antibodies bound to glomeruli occurred in vitro. The findings suggest the presence of subpopulations of anti-ds-DNA antibodies which preferentially bind to glomeruli containing immune complexes.