Photoprotective Acclimation of the Arabidopsis thaliana Leaf Proteome to Fluctuating Light
Photoprotective Acclimation of the Arabidopsis thaliana Leaf Proteome to Fluctuating Light
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拟南芥叶蛋白质组对波动光的光保护适应
DOI：
10.3389/fgene.2020.00154
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发表时间：
2020-03
影响因子：
3.7
通讯作者：
Stefan Niedermaier;Trang Schneider;Marc-Oliver Bahl;S. Matsubara;Pitter F. Huesgen
中科院分区：
文献类型：
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作者：
Stefan Niedermaier;Trang Schneider;Marc-Oliver Bahl;S. Matsubara;Pitter F. Huesgen
植物在其自然生长环境中受到强烈的光强波动，这是由于天气条件和云层和上层冠层叶子的运动引起的不可预测的变化以及昼夜循环中可预测的变化造成的。长期适应波动光（FL）的机制尚不清楚。在这里，我们使用定量质谱法研究了低光生长的拟南芥对FL条件的长期驯化，该条件诱导轻度光氧化应激。在暴露于FL的第三天，在早上和一天结束时收获幼叶和成熟叶，使用稳定同位素标记方法进行蛋白质组学分析。我们鉴定了2,313种蛋白质，其中559种蛋白质在四个实验组（早上-年轻，早上-成熟，结束-年轻，结束-成熟）中的至少一个组中表现出显著的丰度变化。在三个或四个实验组中，49个核心蛋白质组对FL表现出显著的响应，其中包括参与光保护、光系统I周围循环电子流、光呼吸和糖酵解的蛋白质积累增强，而特定的谷胱甘肽转移酶和参与翻译和叶绿素生物合成的蛋白质丰度降低。此外，我们主要在一天结束时观察到通路和蛋白质特异性变化，而仅在早上观察到很少的变化。将蛋白质组数据与匹配的转录数据进行比较，揭示了基因和蛋白质特异性反应，尽管在FL下基因表达增加，但一些叶绿体定位的蛋白质丰度减少。总之，我们的数据显示，在适应FL过程中，蛋白质丰度发生了适度但广泛的改变，并表明蛋白质丰度的转录后调控起着重要作用。
Plants are subjected to strong fluctuations in light intensity in their natural growth environment, caused both by unpredictable changes due to weather conditions and movement of clouds and upper canopy leaves and predictable changes during day-night cycle. The mechanisms of long-term acclimation to fluctuating light (FL) are still not well understood. Here, we used quantitative mass spectrometry to investigate long-term acclimation of low light-grown Arabidopsis thaliana to a FL condition that induces mild photooxidative stress. On the third day of exposure to FL, young and mature leaves were harvested in the morning and at the end of day for proteome analysis using a stable isotope labeling approach. We identified 2,313 proteins, out of which 559 proteins exhibited significant changes in abundance in at least one of the four experimental groups (morning-young, morning-mature, end-of-day-young, end-of-day-mature). A core set of 49 proteins showed significant responses to FL in three or four experimental groups, which included enhanced accumulation of proteins involved in photoprotection, cyclic electron flow around photosystem I, photorespiration, and glycolysis, while specific glutathione transferases and proteins involved in translation and chlorophyll biosynthesis were reduced in abundance. In addition, we observed pathway- and protein-specific changes predominantly at the end of day, whereas few changes were observed exclusively in the morning. Comparison of the proteome data with the matching transcript data revealed gene- and protein-specific responses, with several chloroplast-localized proteins decreasing in abundance despite increased gene expression under FL. Together, our data shows moderate but widespread alterations of protein abundance during acclimation to FL and suggests an important role of post-transcriptional regulation of protein abundance.