Monitoring urinary mercapturic acids as biomarkers of human dietary exposure to acrylamide in combination with acrylamide uptake assessment based on duplicate diets
Monitoring urinary mercapturic acids as biomarkers of human dietary exposure to acrylamide in combination with acrylamide uptake assessment based on duplicate diets
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DOI：
10.1007/s00204-015-1494-9
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发表时间：
2016-04-01
影响因子：
6.1
通讯作者：
Richling, Elke
中科院分区：
文献类型：
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作者：
Ruenz, Meike;Bakuradze, Tamara;Richling, Elke
本人为干预研究调查了丙烯酰胺（AA）含量最低、低和高的饮食摄入与尿暴露生物标志物水平之间的关系。作为生物标志物，监测巯基酸、n -乙酰基- s -（氨甲酰乙基）-l-半胱氨酸（AAMA）和n -乙酰基- s -（1-氨甲酰-2-羟乙基）-l-半胱氨酸（GAMA）。14名健康男性志愿者参加了为期9天的研究，在控制条件下排除了任何无意的AA暴露。通过测定志愿者所吃的所有食物的AA含量来测量饮食中AA的含量。研究设计包括3天的AA最小化饮食初始洗脱期，使饮食中AA的暴露量不超过41 ng/kg bw/d。AA暴露后各2天相同的洗脱期（第4天，低暴露，第7天，高暴露）。在各自的AA摄入日，志愿者从食物中摄入0.6-0.8 μ g AA（低暴露）或1.3-1.8 μ g AA（高暴露）/kg bw/d。低AA和高AA摄入量在72小时内的AAMA输出量分别为各自AA摄入量的58%。在最初的3天洗脱期结束时，记录的AAMA基线水平为93 +/- a31 nmol/d，表明假设的净AA基线暴露水平为0.2-0.3 μ g AA/kg bw/d。
The present human intervention study investigated the relation between the intake of acrylamide (AA) in diets with minimized, low, and high AA contents and the levels of urinary exposure biomarkers. As biomarkers, the mercapturic acids, N-acetyl-S-(carbamoylethyl)-l-cysteine (AAMA), and N-acetyl-S-(1-carbamoyl-2-hydroxyethyl)-l-cysteine (GAMA) were monitored. The study was performed with 14 healthy male volunteers over a period of 9 days, under controlled conditions excluding any inadvertent AA exposure. Dietary exposure to AA was measured by determining AA contents in duplicates of all meals consumed by the volunteers. The study design included an initial washout period of 3 days on AA-minimized diet, resulting in dietary AA exposure not exceeding 41 ng/kg bw/d. Identical washout periods of 2 days each followed the AA exposure days (day 4, low exposure, and day 7, high exposure). At the respective AA intake days, volunteers ingested 0.6-0.8 (low exposure) or 1.3-1.8 (high exposure) mu g AA/kg bw/d with their food. Both low and high AA intakes resulted in an AAMA output within 72 h corresponding to 58 % of the respective AA intake. At the end of the initial 3-day washout period, an AAMA baseline level of 93 +/- A 31 nmol/d was recorded, suggestive for an assumed net AA baseline exposure level of 0.2-0.3 mu g AA/kg bw/d.