Preservation of donor cornea prevents corneal allograft rejection by inhibiting induction of alloimmunity
Preservation of donor cornea prevents corneal allograft rejection by inhibiting induction of alloimmunity
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DOI：
10.1006/exer.2000.0841
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发表时间：
2000-06-01
影响因子：
3.4
通讯作者：
Yamagami, S
中科院分区：
文献类型：
--
作者：
Kamiya, K;Hori, J;Yamagami, S
为了确定供体角膜的保存是否防止同种异体移植物排斥，使用保存在储存介质（Optisol-GS（R））中的角膜进行原位角膜移植。从C3 H/He（H-2（k））小鼠和B10.D2（H-2（d））小鼠收获的供体角膜在储存培养基中保存0、3、7和14天，然后移植到受体BALB/c（H-2（d））小鼠的角膜床中。移植物存活率由临床和组织学确定。用免疫组化和免疫印迹法分析保存角膜中主要组织相容性复合物（MHC）分子的表达。移植后3周评估供体特异性细胞毒性T淋巴细胞（CTL）和迟发型超敏反应（DTH）。移植后3周，还检查了受体小鼠中DTH的主动抑制。保存14天的同种异体移植物在MHC和次要组织相容性（H）抗原以及仅次要H抗原不同组合方面的存活率均显著高于未保存的同种异体移植物。保存同种异体移植物的受体不能诱导CTL和DTH。DTH的主动抑制在这些受者中未获得。供体来源的MHC I类抗原的表达显着减少保存后的角膜。保存供体角膜对预防角膜移植排斥反应有显著效果。由于保存的同种异体角膜移植物不能诱导供体特异性CTL和DTH，并且受者也没有获得对DTH的主动抑制，因此，同种异体角膜移植排斥反应的预防是由于同种异体角膜移植物致敏失败所致。这些结果表明，保存的角膜移植物中MHC I类抗原和次要H抗原表达的减少导致了同种异体角膜移植物致敏失败，从而导致了角膜移植物的高接受率。(C)北京大学出版社.
To determine whether preservation of the donor cornea prevents allograft rejection, orthotopic corneal transplantation was performed using corneas preserved in storage medium (Optisol-GS(R)). Donor corneas harvested from C3H/He (H-2(k)) mice and B10.D2 (H-2(d)) mice were preserved in storage medium for 0, 3, 7 and 14 days, and then transplanted into the corneal beds of the recipient BALB/c (H-2(d)) mice. Graft survival was determined clinically and histologically. The expression of major histocompatibility complex (MHC) molecules in the preserved corneas was analysed by immunohistochemistry and Western blotting. Donor-specific cytotoxic T lymphocyte (CTL) and delayed-type hypersensitivity (DTH) responses were assessed 3 weeks after grafting. Active suppression of DTH in the recipient mice was also examined 3 weeks after grafting. The survival of 14 day preserved allografts was significantly higher than that of the non-preserved allografts in both MHC and minor histocompatibility (H) antigens, and minor H only disparate combination. The recipients of the preserved allografts failed to induce both CTL and DTH. The active suppression of DTH was not acquired in these recipients. The expression of donor-derived MHC class I antigens was markedly reduced in the corneas after preservation. Preservation of the donor cornea had a remarkable effect on the prevention of corneal allograft rejection. Since the preserved allografts failed to induce donor-specific CTL and DTH, and active suppression of DTH was not acquired in the recipients, the prevention of allo-rejection is due to a failure of allo-sensitization, These results indicate that the reduction of MHC class I antigens and minor H antigens expression in the preserved grafts induces a failure of allo-sensitization and leads to the high rate of acceptance in corneal allografts. (C) 2000 Academic Press.