Induction of c-fos during myelomonocytic differentiation and macrophage proliferation
Induction of c-fos during myelomonocytic differentiation and macrophage proliferation
复制标题
骨髓单核细胞分化和巨噬细胞增殖过程中 c-fos 的诱导
作者：
R. Müller;T. Curran;D. Müller;L. Guilbert
先前的研究表明，c-fos在胚胎膜1 - 3、造血细胞4、5和畸胎瘤干细胞6的细胞分化中起作用。在其他细胞类型中，如成纤维细胞，c-fos的表达通常非常低，但可被肽生长因子迅速诱导，暗示c-fos参与生长控制机制7 - 10。在这里，我们发现TPA （12- o - tetradecanoylpholl -13-acetate）诱导的HL60人早幼粒细胞前体细胞巨噬细胞样分化11,12在治疗后15分钟内伴随着c-fos mRNA和蛋白的诱导，提示c-fos在该分化系统中具有功能作用。在静止的终分化巨噬细胞中，c-fos的表达可被巨噬细胞特异性生长因子集落刺激因子-1 （CSF-1）诱导13。然而，c-fos诱导的动力学与生长因子刺激的成纤维细胞完全不同，这支持了c-fos基因产物在不同细胞类型中可能具有不同功能的观点。
Previous studies have suggested a role for c-fos in cellular differentiation in fetal membranes1–3, haematopoietic cells4,5 and teratocarcinoma stem cells6. In other cell types, such as fibroblasts, c-fos expression is normally very low, but is rapidly induced by peptide growth factors, implicating c-fos in growth control mechanisms7–10. Here, we show that the TPA (12-O-tetradecanoylphorbol-13-acetate)-induced macrophage-like differentiation of HL60 human promyelocytic precursor cells11,12 is accompanied by the induction of both c-fos mRNA and protein within 15 min after treatment, suggesting a functional role for c-fos in this differentiation system. In quiescent terminally differentiated macrophages, expression of c-fos is inducible by the macrophage-specific growth factor colony-stimulating factor-1 (CSF-1)13. The kinetics of c-fos induction, however, are entirely different from those in growth factor-stimulated fibroblasts, supporting the view that the c-fos gene product may serve different functions in different cell types.
集落刺激因子 (CSF-1) 的纯化、测定、表征和靶细胞结合的方法。
影响因子：
2.2
作者：
Stanley,ER;Guilbert,LJ
通讯作者：
Guilbert,LJ