In vitro degradation of novel bioactive polycaprolactone-20% tricalcium phosphate composite scaffolds for bone engineering
In vitro degradation of novel bioactive polycaprolactone-20% tricalcium phosphate composite scaffolds for bone engineering
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DOI：
10.1016/j.msec.2006.05.006
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发表时间：
2007-03-01
期刊：
影响因子：
--
通讯作者：
Teoh, S. H.
中科院分区：
文献类型：
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作者：
Lei, Y.;Rai, B.;Teoh, S. H.
本课题组最近制备了新型三维聚己内酯-20%磷酸三钙（PCL-TCP）复合支架材料，用于骨工程。此类合成生物材料的生物活性可以通过检查其浸入模拟体液（SBF）中时引发其表面形成磷灰石的能力来评估。在这项研究中，这些支架的体外降解行为进行了系统的监测，在不同的时间段的1，7，14，21和28天后，浸泡在SBF在37 ℃。样品的重量损失和吸水率表明PCL-TCP支架仅缓慢降解。生化分析和pH值的测量显示，羟基磷灰石，骨的主要无机成分，开始形成后17天浸泡在SBF的支架表面。Von Kossa实验表明，在SBF中浸泡2周后，支架表面的钙沉积进行性增加。扫描电子显微镜验证了在整个时间段内形成的磷灰石层的表面结晶。总之，PCL与TCP在复合支架中的协同作用赋予了生物可吸收性和生物活性，这为骨再生目的提供了一种令人兴奋的方法。(c)2006年由Elsevier B. V.出版
Our group recently fabricated novel 3D polycaprolactone-20% tricalcium phosphate (PCL-TCP) composite scaffolds for applications in bone engineering. The bioactivity of such synthetic biomaterials can be evaluated by examining its ability to initiate the formation of apatite on its surface when immersed in simulated body fluids (SBF). In this study, the in vitro degradation behaviors of these scaffolds were systematically monitored for varying time periods of 1, 7, 14, 21 and 28 days post-immersion in SBF at 37 degrees C. Weight loss and water absorption of the samples indicated that PCL-TCP scaffolds were only slowly degraded. Biochemical assays and pH measurements revealed that hydroxyapatite, the main inorganic constituent of bone, commenced to form on the surface of the scaffolds after 17 days of immersion in SBF. Von Kossa assays demonstrated that calcium deposits increased progressively on the surface of the scaffolds after soaking in SBF for 2 weeks. Scanning electron microscopy verified the surface crystallization of the apatite layer formed over the entire period of time. In conclusion, the synergy of PCL with TCP in a composite scaffold confers both bioresorbability as well as bioactivity that offer an exciting approach for bone regeneration purposes. (c) 2006 Published by Elsevier B.V.