Identification of T Peripheral Helper (Tph) Cells.
Identification of T Peripheral Helper (Tph) Cells.
复制标题
外周辅助 T (Tph) 细胞的鉴定。
DOI：
10.1007/978-1-0716-1736-6_6
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Rao,DeepakA
中科院分区：
文献类型：
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作者：
Wacleche,VanessaS;Wang,Runci;Rao,DeepakA
T外周辅助细胞（T peripheral helper, Tph）是一种刺激外周组织内B细胞反应的T细胞群。与T滤泡辅助细胞（Tfh）一样，PD-1hiCXCR5−CD4+Tph细胞也产生IL-21和CXCL13，但这些细胞在转录因子和趋化因子受体的表达上与Tfh细胞不同。最初在类风湿关节炎滑膜中发现的Tph细胞在多种自身免疫性疾病中扩增。Tph细胞可以通过流式细胞术和质量细胞术与Tfh细胞鉴别。在这里，我们描述了详细的方法来鉴定Tph细胞和表征相关的表型特征和功能。这些方案和附加说明为确定和探索Tph细胞在各种人类疾病中的作用提供了指南。
T peripheral helper (Tph) cells represent a T cell population that stimulates B cell responses within peripheral tissues. Like T follicular helper (Tfh) cells, PD-1hiCXCR5−CD4+Tph cells produce IL-21 and CXCL13, yet these cells differ from Tfh cells in expression of both transcription factors and chemokine receptors. Originally identified in rheumatoid arthritis synovium, Tph cells are expanded in multiple autoimmune diseases. Tph cells can be identified and distinguished from Tfh cells by both flow cytometry and mass cytometry. Here, we describe detailed methods to identify Tph cells and characterize associated phenotypic features and functions. These protocols and additional notes provide a guide to identify and explore the roles of Tph cells in various human diseases.