IMMUNOGLOBULIN CHAIN LOSS IN HYBRIDOMA LINES
IMMUNOGLOBULIN CHAIN LOSS IN HYBRIDOMA LINES
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DOI：
10.1073/pnas.77.4.2197
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发表时间：
1980-01-01
期刊：
影响因子：
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通讯作者：
KOHLER, G
中科院分区：
文献类型：
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作者：
KOHLER, G
构建分泌一种、两种或三种不同免疫球蛋白的杂交细胞。监测免疫球蛋白重链或轻链表达的损失。链损失是随机的，只有在线与过量的活性轻链基因超过重链基因。在所有其他组合中，观察到优先的重链丢失。重链或轻链合成改变的变体细胞表现出改变的链丢失模式。因此，提出游离免疫球蛋白重链对细胞是有毒的。这两种基因产物的相互依赖性为杂交细胞中明显的定向染色体丢失提供了可能的分子解释。
Hybrid cells secreting one, two, or three different immunoglobulins were constructed. The loss of immunoglobulin heavy or light chain expression was monitored. Chain loss was random only in lines with an excess of active light chain genes over heavy chain genes. In all other combinations preferential heavy chain loss was observed. Variant cells altered in heavy or light chain synthesis exhibited an altered chain loss pattern. It is therefore proposed that free immunoglobulin heavy chain is toxic for the cells. The interdependence of the two gene products gives a possible molecular explanation of apparent directed chromosome loss in hybrid cells.