Lithium-Incorporated Nanoporous Coating Formed by Micro Arc Oxidation (MAO) on Magnesium Alloy with Improved Corrosion Resistance, Angiogenesis and Osseointegration
Lithium-Incorporated Nanoporous Coating Formed by Micro Arc Oxidation (MAO) on Magnesium Alloy with Improved Corrosion Resistance, Angiogenesis and Osseointegration
复制标题
通过微弧氧化（MAO）在镁合金上形成的掺锂纳米孔涂层具有改善的耐腐蚀性、血管生成和骨整合
DOI：
10.1166/jbn.2019.2767
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发表时间：
2019-06-01
影响因子：
2.9
通讯作者：
Zhang, Xianlong
中科院分区：
文献类型：
--
作者：
Liu, Wei;Li, Tingting;Zhang, Xianlong
镁是一种有吸引力的候选人，骨科植入物，由于其类似的机械性能，人类骨和生物降解性。但镁的高腐蚀性、高pH值、成骨活性差等问题阻碍了其进一步的应用。本研究采用微弧氧化技术在AZ 91镁合金表面引入纳米多孔涂层，提高其耐蚀性。同时，我们通过在含锂电解液中反应，向涂层中添加了一种血管生成和成骨元素锂（命名为Li-MAO），以同时增强AZ 91的血管生成和成骨活性。电化学测试和浸泡实验表明，Li-MAO具有良好的耐腐蚀性能。通过CCK-8细胞计数试剂盒和活/死试验，观察到Li-MAO样品具有更好的生物相容性。免疫荧光染色、茜素红染色及成骨相关基因表达的体外实验结果表明，Li-MAO组具有较好的成骨能力，这可能与激活Wnt/beta-catenin通路有关。体外血管生成实验也显示Li-MAO组血管生成更好。建立骨缺损修复模型，评价样品的体内骨整合情况。Micro-CT扫描、荧光染料序贯标记和货车Gieson染色结果表明，Li-MAO的体内骨修复能力优于其他组。体内、体外实验结果表明，含锂纳米多孔涂层能够提高镁合金的耐腐蚀性、血管生成能力和骨结合能力，为锂微弧氧化镁合金的临床应用提供了新的思路。
Magnesium is an attractive candidate for orthopedic implants due to its similar mechanical properties to human bone and biodegradability. However, the high corrosion rate of magnesium accompanying with high pH value and poor osteogenic activity hinder its further application. In this study. we employed micro arc oxidation (MAO) to improve corrosion resistance by introduce nanoporous coating on AZ91 magnesium. Meanwhile, we added lithium, an angiogenic and osteogenic element, to the coating (named as Li-MAO) by reaction in a lithium-containing electrolyte to simultaneously enhance angiogenic and osteogenic activity of AZ91. The favorable corrosion resistance of Li-MAO was verified by electrochemical and immersion test in vitro. Better biocompatibility was observed in Li-MAO samples by cell counting kit-8 (CCK-8) and live/dead assay. The in vitro results of immunofluorescence staining, Alizarin red staining and osteogenic-related genes expression indicated better osteogenic ability of Li-MAO group, which may result from the activation the Wnt/beta-catenin pathway. In vitro angiogenic tests also demonstrated better angiogenesis in Li-MAO group. A bone defect repair model was built to evaluate the in vivo osseointegration of samples. The outcomes of micro-computed tomography (Micro-CT) scanning, sequential fluorochrome labeling and Van Gieson staining suggested better in vivo bone repair ability of Li-MAO than other groups. Our promising results both in vitro and in vivo demonstrated that lithium-containing nanoporous coating can improve corrosion resistance, angiogenesis and osseointegration of magnesium alloy, which may forward the clinic application of Li-MAO magnesium.