APP(DeltaNL695) expression in murine tissue downregulates CNBP expression.
APP(DeltaNL695) expression in murine tissue downregulates CNBP expression.
复制标题
小鼠组织中的 APP(DeltaNL695) 表达下调 CNBP 表达。
DOI：
10.1016/j.neulet.2010.07.006
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发表时间：
2010
影响因子：
2.5
通讯作者：
Murphy,MPaul
中科院分区：
文献类型：
--
作者：
Niedowicz,DanaM;Beckett,TinaL;Holler,ChrisJ;Weidner,AdamM;Murphy,MPaul
细胞核酸结合蛋白（CNBP）是一种广泛表达的蛋白质，参与转录和翻译的调控。CNBP及其编码基因ZNF 9已被证明参与2型强直性肌营养不良。阿尔茨海默病（AD）和散发性包涵体肌炎（sIBM）都是与β-淀粉样蛋白积累相关的年龄相关性退行性疾病。在小鼠中过表达淀粉样前体蛋白（APP）已被用于产生这两种疾病的模型。我们在这里表明，APP在骨骼肌中的过度表达，从小鼠模型sIBM降低CNBP的表达显着。我们检测了CNBP在脑特异性APP过表达菌株和全身APP敲入菌株中的表达，发现在表达APPSwe的组织中CNBP表达减少。我们的结论是APPSwein小鼠组织中的表达诱导CNBP表达的减少。这种效应似乎不是由于CNBP转录的改变。APPSW表达可能为CNBP调控的研究提供工具，并为这两种蛋白在疾病中的作用提供线索。
The cellular nucleic acid binding protein (CNBP) is a ubiquitously expressed protein involved in regulation of transcription and translation. CNBP, and its encoding gene ZNF9, have been shown to be involved in type 2 myotonic dystrophy. Both Alzheimer's disease (AD) and sporadic inclusion body myositis (sIBM) are age-related degenerative diseases associated with the accumulation of β-amyloid. Overexpression of amyloid precursor protein (APP) in mice has been used to generate models of both diseases. We show here that overexpression of APP in skeletal muscle from a mouse model of sIBM reduces the expression of CNBP significantly. We examined CNBP expression in a brain-specific APP-overexpressing strain, and a whole body APP knock-in strain, and found that there was a reduction in CNBP expression in tissue expressing APPSwe. We conclude that expression of APPSwein murine tissue induces a decrease in CNBP expression. This effect does not appear to be due to alterations in CNBP transcription. APPSweexpression may provide a tool for the study of CNBP regulation and clues to the roles of both proteins in disease.