A CYCLIC PATHWAY FOR THE BACTERIAL DISSIMILATION OF 2,3-BUTANEDIOL, ACETYLMETHYLCARBINOL, AND DIACETYL .1. GENERAL ASPECTS OF THE 2,3-BUTANEDIOL CYCLE
A CYCLIC PATHWAY FOR THE BACTERIAL DISSIMILATION OF 2,3-BUTANEDIOL, ACETYLMETHYLCARBINOL, AND DIACETYL .1. GENERAL ASPECTS OF THE 2,3-BUTANEDIOL CYCLE
复制标题
DOI：
10.1128/jb.71.4.425-432.1956
复制
发表时间：
1956-01-01
影响因子：
3.2
通讯作者：
HEYM, GA
中科院分区：
文献类型：
--
作者：
JUNI, E;HEYM, GA
能够以 2、3 丁二醇或乙酰甲基甲醇作为 C 的唯一来源生长的细菌形成了将这些化合物转化为乙酸盐的循环机制。二乙酰是该过程中的中间体，通过二磷酸硫胺催化的偶姻缩合反应转化为二乙酰甲基甲醇 ((CH3CO)2(CH3)COH) 和乙酸。二乙酰基甲基甲醇被还原为乙酰基丁二醇 (CH3 CHOHCOH(COCH3)CH3)，乙酰基丁二醇又被水解为 2,3-丁二醇和乙酸酯。循环中的所有反应都可以在无氧条件下进行。有证据表明，该循环过程在 4-C 化合物的正常需氧生长过程中进行。描述了二乙酰甲基甲醇和乙酰丁二醇（在 Voges-Proskauer 试验中作为乙酰甲基甲醇反应的化合物）的测定方法。
Bacteria able to grow with 2, 3 butanediol or acetylmethylcarbinol as the sole source of C develop a cyclic mechanism for the conversion of these compounds to acetate. Diacetyl is an intermediate in the process and is converted to diacetylmethylcarbinol ((CH3CO)2(CH3)COH) and acetate by a diphosphothiamin-catalyzed acyloin condensation reaction. Diacetylmethylcarbinol is reduced to acetylbutanediol (CH3 CHOHCOH(COCH3)CH3) which, in turn, is hydrolyzed to 2,3-butanediol and acetate. All the reactions in the cycle can take place anaerobically. Evidence is presented to show that this cyclic process operates during normal aerobic growth on the 4-C compounds. Methods for determination of diacetylmethylcarbinol and acetylbutanediol, compounds which react as acetylmethylcarbinol in the Voges-Proskauer test, are described.