Differential membrane protein carboxyl-methylation of intact human erythrocytes by exogenous methyl donors.
Differential membrane protein carboxyl-methylation of intact human erythrocytes by exogenous methyl donors.
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外源甲基供体对完整人红细胞的差异膜蛋白羧甲基化。
DOI：
10.1042/bj2190743
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Kim,S
中科院分区：
文献类型：
--
作者：
Ro,JY;DiMaria,P;Kim,S
在完整的人红细胞中，通过蛋白甲基化酶II（S-腺苷甲硫氨酸：蛋白羧基O-甲基转移酶，EC 2.1.1.24）进行的膜蛋白羧基甲基化模式显示出显著不同，无论甲基供体S-腺苷-L-[甲基-3H]甲硫氨酸是外源提供的还是通过外源添加的L-[甲基-3H]甲硫氨酸在细胞内形成的。差异包括以下方面。(1)细胞骨架成分（条带2.1和4.1）的甲基化仅发生在L-[甲基-3H]甲硫氨酸标记的细胞中。(2)甲硫氨酸介导的甲基化对S-腺苷-L-同型半胱氨酸抑制的敏感性远低于腺苷甲硫氨酸介导的甲基化（10 μ M时抑制率为22%对95%）。(3)由外源腺苷甲硫氨酸和甲硫氨酸介导的膜蛋白甲基化在它们的碱不稳定性方面显著不同;在pH 6.0下，30%的腺苷甲硫氨酸介导的蛋白甲酯在30分钟（37 ° C）后水解，而甲硫氨酸介导的酯是稳定的。在pH7.4时，孵育30分钟的不稳定性分别为60%和30%。为了解释这些结果，与完整的红细胞相关的细胞骨架结构的可能参与进行了讨论。
The patterns of membrane protein carboxyl-methylation by protein methylase II (S-adenosylmethionine: protein-carboxyl O-methyltransferase, EC 2.1.1.24) in intact human erythrocytes were shown to differ markedly whether the methyl donor, S-adenosyl-L-[methyl-3H]methionine, was supplied exogenously or formed intracellularly via exogenously added L-[methyl-3H]methionine. The differences include the following. (1) The methylation of cytoskeletal components (band 2.1 and 4.1) occurs only in the case of the L-[methyl-3H]methionine-labelled cells. (2) The methionine-mediated methylation was much less sensitive to S-adenosyl-L-homocysteine inhibition than the adenosylmethionine-mediated methylation (22% versus 95% inhibition at 10 microM). (3) The membrane protein methylation mediated by exogenous adenosylmethionine and methionine differed markedly in their alkali labilities; at pH 6.0, 30% of the adenosylmethionine-mediated protein methyl esters were hydrolysed after 30 min (37 degrees C) while the methionine-mediated esters were stable. At pH 7.4, the respective labilities were 60% and 30% for the 30 min incubation. To explain these results, a possible involvement of cytoskeletal structure associated with the intact erythrocyte is discussed.
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