Dynamics of Filamentous Viruses in Polyelectrolyte Solutions
Dynamics of Filamentous Viruses in Polyelectrolyte Solutions
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DOI：
10.1021/acs.macromol.2c01641
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发表时间：
2022-11
期刊：
影响因子：
5.5
通讯作者：
Farshad Safi Samghabadi;Ali H. Slim;Maxwell Smith;Maede Chabi;J. Conrad
中科院分区：
文献类型：
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作者：
Farshad Safi Samghabadi;Ali H. Slim;Maxwell Smith;Maede Chabi;J. Conrad
聚电解质的结构和动力学不同于中性聚合物。这些差异如何影响各向异性颗粒的传输仍然没有完全理解。在这里，我们调查运输的半柔性M13噬菌体（噬菌体）的聚苯乙烯磺酸钠（PSS）与各种离子强度的半稀释水溶液中使用荧光显微镜。我们使用68和2200 kDa的两种分子量，并通过将溶液的离子强度从10 − 6 M变化到10 − 1 M来调整PSS的特征长度尺度。噬菌体在所有聚合物浓度下均表现出扩散动力学。对于2200 kDa的PSS解决方案，噬菌体动力学单调偏离批量预测的聚合物浓度的增加，并表现出非高斯分布的位移。现有的缩放理论可以近似地将动力学作为噬菌体流体动力学半径与聚合物尺寸比R h /m的函数折叠到跨聚合物浓度和离子强度的主曲线上。然而，这种部分崩溃，不遵循各向同性颗粒在柔性高斯链中的扩散的预测，这表明存在多个扩散模式，由于各向异性结构的噬菌体和封闭的长度尺度设置的结构和动力学的带电聚合物。
: The structure and dynamics of polyelectrolytes differ from those of neutral polymers. How these differences affect the transport of anisotropic particles remains incompletely understood. Here, we investigate the transport of semiflexible M13 bacteriophage (phage) in aqueous semidilute solutions of sodium polystyrenesulfonate (PSS) with various ionic strengths using fluorescence microscopy. We tune the characteristic length scales of the PSS using two molecular weights of 68 and 2200 kDa and by varying the ionic strength of the solutions from 10 − 6 to 10 − 1 M. Phage exhibit diffusive dynamics across all polymer concentrations. For 2200 kDa PSS solutions, the phage dynamics monotonically deviate from the bulk prediction as polymer concentration increases and exhibit non-Gaussian distributions of displacements. Existing scaling theories can approximately collapse dynamics as a function of phage hydrodynamic radius to polymer size ratio R h / ξ onto a master curve across polymer concentrations and ionic strengths. This partial collapse, however, does not follow the prediction for diffusion of isotropic particles in flexible Gaussian chains, suggesting the presence of multiple diffusive modes due to the anisotropic structure of the phage and the confining length scales set by the structure and dynamics of charged polymers.