THE REGULATED EXPRESSION OF ERYTHROPOIETIN BY 2 HUMAN HEPATOMA-CELL LINES
THE REGULATED EXPRESSION OF ERYTHROPOIETIN BY 2 HUMAN HEPATOMA-CELL LINES
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DOI：
10.1073/pnas.84.22.7972
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发表时间：
1987-11-01
影响因子：
11.1
通讯作者：
BUNN, HF
中科院分区：
文献类型：
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作者：
GOLDBERG, MA;GLASS, GA;BUNN, HF
细胞培养系统的发展，产生红细胞生成素（Epo）的调节方式一直是许多努力的焦点。我们筛选了多种肾和肝细胞系（包括MDCK、LLC-PK1、BHK、WRL 68、CLCL、A704、CRFK、A498、ACHN、TCMK-1、LLC-MK2、CaKi-2、HepG2和Hep3B）的Epo组成或调控表达。通过放射免疫测定和体外生物测定，只有人肝癌细胞系Hep3B和HepG2产生了大量的Epo（24小时内每106个细胞产生330毫单位）。在两种细胞系中，Epo的组成量随着细胞密度的增加而显著增加。在细胞密度下
The development of a cell culture system that produces erythropoietin (Epo) in a regulated manner has been the focus of much effort. We have screened multiple renal and hepatic cell lines (including MDCK, LLC-PK1, BHK, WRL 68, CLCL, A704, CRFK, A498, ACHN, TCMK-1, LLC-MK2, CaKi-2, HepG2, and Hep3B) for either constitutive or regulated expression of Epo. Only the human hepatoma cell lines, Hep3B and HepG2, made significant amounts of Epo as measured both by radioimmunoassay and in vitro bioassay (as much as 330 milliunits per 106 cells in 24 hr). The constitutive production of Epo increased dramatically as a function of cell density in both cell lines. At cell densities