Oxidative Modification of Tryptophan 43 in the Heme Vicinity of the F43W/H64L Myoglobin Mutant*
Oxidative Modification of Tryptophan 43 in the Heme Vicinity of the F43W/H64L Myoglobin Mutant*
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F43W/H64L 肌红蛋白突变体血红素附近色氨酸 43 的氧化修饰*
DOI：
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发表时间：
2001
影响因子：
4.8
通讯作者：
Y. Watanabe
中科院分区：
文献类型：
--
作者：
I. Hara;T. Ueno;S. Ozaki;S. Itoh;Keonil Lee;N. Ueyama;Y. Watanabe
The F43W/H64L myoglobin mutant was previously constructed to investigate the effects of electron-rich tryptophan residue in the heme vicinity on the catalysis, where we found that Trp-43 in the mutant was oxidatively modified in the reaction with m-chloroperbenzoic acid (mCPBA). To identify the exact structure of the modified tryptophan in this study, the mCPBA-treated F43W/H64L mutant has been digested stepwise with Lys-C achromobacter and trypsin to isolate two oxidation products by preparative fast protein liquid chromatography. The close examinations of the 1H NMR spectra of peptide fragments reveal that two forms of the modified tryptophan must have 2,6-disubstituted indole substructures. The 13C NMR analysis suggests that one of the modified tryptophan bears a unique hydroxyl group in stead of the NH2 group at the amino-terminal. The results together with mass spectrometry (MS)/MS analysis (30 Da increase in mass of Trp-43) indicate that oxidation products of Trp-43 are 2,6-dihydro-2,6-dioxoindole and 2,6-dihydro-2-imino-6-oxoindole derivatives. Our finding is the first example of the oxidation of aromatic carbons by the myoglobin mutant system.
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DOI：
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发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Ikeda-Saito,M;Hori,H;Andersson,LA;Prince,RC;Pickering,IJ;George,GN;Sanders2nd,CR;Lutz,RS;McKelvey,EJ;Mattera,R
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Mattera,R
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DOI：
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发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
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作者：
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通讯作者：
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DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Morikis,D;Champion,PM;Springer,BA;Egebey,KD;Sligar,SG
通讯作者：
Sligar,SG