Remarkable sequence conservation of transcripts encoding amphibian and mammalian homologues of quaking, a KH domain RNA-binding protein.
Remarkable sequence conservation of transcripts encoding amphibian and mammalian homologues of quaking, a KH domain RNA-binding protein.
复制标题
DOI：
10.1016/s0378-1119(96)00795-0
复制
发表时间：
1997-04
期刊：
影响因子：
3.5
通讯作者：
A. Zorn;M. Grow;K. Patterson;T. Ebersole;Q. Chen;K. Artzt;P. Krieg
中科院分区：
文献类型：
--
作者：
A. Zorn;M. Grow;K. Patterson;T. Ebersole;Q. Chen;K. Artzt;P. Krieg
小鼠震颤位点的突变可导致两种不同类型的发育表型：（1）中枢神经系统髓鞘缺乏，伴有特征性震颤，或（2）妊娠第9天左右的胚胎致死。一个编码KH基序蛋白的候选基因（qkI）最近被鉴定出来。我们已经分离和鉴定编码非洲爪蟾震动同源物（Xqua）的cDNA，并从表达的序列标签中组装了一个几乎完整的人类震动序列。序列比较表明，两栖动物和哺乳动物的quaking转录本显示出惊人的保守性，无论是在编码区，并出乎意料地，在3′ UTR。两个Xqua成绩单5 kb和5.5 kb的长度在非洲爪蟾胚胎中差异表达，与5 kb的转录检测早在原肠胚发育阶段。使用体外测定，我们已经证明了由5 kb转录物编码的震动蛋白的RNA结合活性。总之，震动序列的高度保守性表明在脊椎动物发育中具有重要的保守功能，可能在RNA代谢的调节中。© 1997 Elsevier Science B.V.保留所有权利。
Mutations in the mouse quaking locus can result in two different types of developmental phenotypes: (1) a deficiency of myelin in the central nervous system that is accompanied by a characteristic tremor, or (2) embryonic lethality around day 9 of gestation. A quaking candidate gene (qkI) that encodes a KH motif protein has recently been identified. We have isolated and characterized cDNAs encoding the Xenopus quaking homologue (Xqua) and also assembled an almost complete human quaking sequence from expressed sequence tags. Sequence comparisons show that the amphibian and mammalian quaking transcripts exhibit striking conservation, both within the coding region and, unexpectedly, in the 3′ UTR. Two Xqua transcripts 5 kb and 5.5 kb in length are differentially expressed in the Xenopus embryo, with the 5 kb transcript being detected as early as the gastrula stage of development. Using an in vitro assay, we have demonstrated RNA-binding activity for quaking protein encoded by the 5 kb transcript. Overall, the high sequence conservation of quaking sequences suggests an important conserved function in vertebrate development, probably in the regulation of RNA metabolism. © 1997 Elsevier Science B.V. All rights reserved.