High-soy diet decreases infarct size after permanent middle cerebral artery occlusion in female rats.
High-soy diet decreases infarct size after permanent middle cerebral artery occlusion in female rats.
复制标题
高大豆饮食可减少雌性大鼠大脑中动脉永久性闭塞后的梗塞面积。
DOI：
10.1152/ajpregu.00642.2004
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Schreihofer,AnnM
中科院分区：
文献类型：
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作者：
Schreihofer,DerekA;Do,KhoiD;Schreihofer,AnnM
在缺血性中风的啮齿动物模型中，雌激素是一种强大的神经保护剂。然而，在人类中，雌激素治疗会增加中风的风险。与激素替代相关的健康风险导致许多女性考虑替代疗法，包括高大豆饮食或含有大豆异黄酮的补充剂，这些大豆异黄酮充当雌激素受体配体，选择性地模拟雌激素的某些作用。我们假设，高大豆饮食将分享雌激素在局灶性脑缺血中的神经保护作用。将雌性SD大鼠摘除卵巢后，随机分为3组：无异黄酮组+安慰剂组(IF-P)、无异黄酮组+雌二醇组(IF-E)、高大豆饲料+安慰剂组(S-P)。喂食2周后，大鼠进行左侧大脑中动脉永久性闭塞(MCAO)。不同组间同侧脑血流量的减少程度相同(∼为50%)。24小时后测定神经功能缺损程度，取脑组织行TTC染色测定脑梗塞程度。在IF-P大鼠，MCAO造成50%±4%的脑梗塞。雌激素和高大豆饮食均能使IF-E大鼠和S-P大鼠的脑梗塞面积分别减少到26±5%和37±5%。在五个吻尾水平的分析表明，雌激素治疗在减少梗塞面积方面比高大豆饮食略有效。24小时的整体神经功能缺失评分与梗塞面积相关；然而，治疗组之间没有统计学上的显著差异。这些数据表明，在大鼠局灶性脑缺血模型中，2wk的高大豆饮食是减少卒中范围的有效预防策略。
Estrogen is a powerful neuroprotective agent in rodent models of ischemic stroke. However, in humans, estrogen treatment can increase risk of stroke. Health risks associated with hormone replacement have led many women to consider alternative therapies including high-soy diets or supplements containing soy isoflavones, which act as estrogen receptor ligands to selectively mimic some of estrogen's actions. We hypothesized that a high-soy diet would share the neuroprotective actions of estrogen in focal cerebral ischemia. Female Sprague-Dawley rats were ovariectomized and divided into three groups: isoflavone-free diet + placebo (IF-P), isoflavone-free diet + estradiol (IF-E), or high-soy diet + placebo (S-P). Two weeks after being placed on diets, rats underwent left permanent middle cerebral artery occlusion (MCAO). Reductions in ipsilateral cerebral blood flow were equivalent across groups (∼50%). Twenty-four hours later neurological deficit was determined, and brains were collected for assay of cerebral infarct by TTC staining. In the IF-P rats MCAO produced a 50 ± 4% cerebral infarct. Estrogen and high-soy diet both significantly reduced the size of the infarcts to 26 ± 5% in IF-E rats and to 37 ± 5% in S-P rats. Analysis at five rostro-caudal levels revealed that estrogen treatment was slightly more effective at reducing infarct size than high soy diet. Overall neurological deficit scores at 24 h correlated with infarct size; however, there were no statistically significant differences among the treatment groups. These data show that 2 wk of a high-soy diet is an effective prophylactic strategy for reducing stroke size in a rat model of focal cerebral ischemia.