Identification of histamine releasing factor(s) in the late phase of cutaneous IgE-mediated reactions.
Identification of histamine releasing factor(s) in the late phase of cutaneous IgE-mediated reactions.
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DOI：
10.4049/jimmunol.136.7.2583
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发表时间：
1986-04
影响因子：
4.4
通讯作者：
Jane Warner;Marek M. Pienkowski;M. Plaut;Philip S. Norman;L. Lichtenstein
中科院分区：
文献类型：
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作者：
Jane Warner;Marek M. Pienkowski;M. Plaut;Philip S. Norman;L. Lichtenstein
我们已经证明，在晚期反应中，从抗原挑战的皮肤水泡收集的液体会导致体外培养的人嗜碱性粒细胞释放大量的组胺(平均值=42%，n=14)。无论是在即刻阶段收集的控制液，还是从未受挑战的水泡收集的控制液，都会从嗜碱性粒细胞和肺肥大细胞中释放小于或等于10%的组胺。晚期水疱液诱导的人肺细胞组胺释放水平较低(平均值=11%，n=4)，略高于但不显著高于对照水泡液诱导的水平。嗜碱性细胞的释放特征在剂量依赖性、钙和温度需求以及动力学方面与IgE介导的刺激相似。通过嗜碱性粒细胞对抗-IgE的脱敏，证明了晚期水疱液对IgE的依赖性，这消除了对抗-IgE和水疱液的反应，但不影响非IgE介导的刺激。用乳酸去除细胞表面的IgE也消除了对抗IgE抗体和晚期水疱液的反应。将“剥离的”细胞与含有IgE骨髓瘤的血清孵育，恢复了对抗-IgE的反应，但不影响对晚期水疱液的反应。利用该因子获得的释放特性与我们小组先前描述的培养的巨噬细胞的IgE依赖性组胺释放因子非常相似。
We have shown that fluids collected from antigen-challenged skin blisters during the late phase reaction cause the release of substantial amounts of histamine (means = 42%, n = 14) from human basophils in vitro. Control fluids collected either during the immediate phase or from an unchallenged blister released less than or equal to 10% histamine from both basophils and lung mast cells. Late phase blister fluids induced low levels of histamine release from human lung cells (means = 11%, n = 4) that were slightly but not significantly greater than levels induced by control blister fluids. The characteristics of basophil release were similar to IgE-mediated stimuli in dose dependence, calcium and temperature requirements, and kinetics. The IgE dependence of the late phase blister fluid was demonstrated by desensitization of the basophils to anti-IgE, which obviated the response to anti-IgE and blister fluid but did not affect a non-IgE-mediated stimulus. Removal of the cell surface IgE with lactic acid also abolished the response to both anti-IgE and late phase blister fluid. Incubation of the "stripped" cells with serum containing IgE myeloma restored the response to anti-IgE but failed to affect response to late phase blister fluid. The characteristics of release obtained with this factor closely resemble those of an IgE-dependent histamine releasing factor from cultured macrophages previously described by our group.