Decoding SARS-CoV-2 Transmission and Evolution and Ramifications for COVID-19 Diagnosis, Vaccine, and Medicine
Decoding SARS-CoV-2 Transmission and Evolution and Ramifications for COVID-19 Diagnosis, Vaccine, and Medicine
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DOI：
10.1021/acs.jcim.0c00501
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发表时间：
2020-12-28
影响因子：
5.6
通讯作者：
Wei, Guo-Wei
中科院分区：
文献类型：
--
作者：
Wang, Rui;Hozumi, Yuta;Wei, Guo-Wei
由于严重的急性急性呼吸综合症冠状病毒2（SARS-COV-2），针对冠状病毒病（CoVID-19）的诊断测试，预防性疫苗和治疗性药物的开发已付出了巨大的努力。这种发展的大部分是基于2020年1月5日收集的参考基因组。基于收集到2020年6月1日收集的15 140个基因组样品的基因分型，我们报告说SARS-COV-2经历了8309个单一突变，可以进行8309个突变。聚集成六个亚型。我们引入了突变比和突变H指数，以表征蛋白质保守性和揭示SARS-COV-2包膜蛋白，主要蛋白酶和内核酸酶蛋白质蛋白相对保守的，而SARS-COV-2核蛋白酶蛋白质，峰值蛋白，帕帕克蛋白，以及Papain-papain--蛋白质和Papain-papain--蛋白质是相对保守的。像蛋白酶相对不保守。特别是，我们已经确定了人口水平的核素基因中40％的核苷酸对核苷酸的突变，信号传导潜在的影响对COVID-19诊断，疫苗，抗体以及抗体和小分子药物的持续发展。
Tremendous effort has been given to the development of diagnostic tests, preventive vaccines, and therapeutic medicines for coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Much of this development has been based on the reference genome collected on January 5, 2020. Based on the genotyping of 15 140 genome samples collected up to June 1, 2020, we report that SARS-CoV-2 has undergone 8309 single mutations which can be clustered into six subtypes. We introduce mutation ratio and mutation h-index to characterize the protein conservativeness and unveil that SARS-CoV-2 envelope protein, main protease, and endoribonuclease protein are relatively conservative, while SARS-CoV-2 nucleocapsid protein, spike protein, and papain-like protease are relatively nonconservative. In particular, we have identified mutations on 40% of nucleotides in the nucleocapsid gene in the population level, signaling potential impacts on the ongoing development of COVID-19 diagnosis, vaccines, and antibody and small-molecular drugs.