br regulates the expression of the ecdysone biosynthesis gene npc1.
br regulates the expression of the ecdysone biosynthesis gene npc1.
复制标题
DOI：
10.1016/j.ydbio.2010.05.510
复制
发表时间：
2010-08
影响因子：
2.7
通讯作者：
Yanhui Xiang;Zhonghua Liu;Xun Huang
中科院分区：
文献类型：
--
作者：
Yanhui Xiang;Zhonghua Liu;Xun Huang
昆虫的生长和变态受环腺产生的蜕皮甾类激素的调节。蜕皮激素生物合成相关基因在环腺中高度特异表达。然而，蜕皮激素生物合成的内在调控很少受到关注。在这里，我们使用果蝇npc1基因研究环腺特异性基因表达的机制。NPC 1对于蜕皮激素生物合成期间在环腺中的固醇运输是重要的。我们已经确定了一个保守的环腺特异性顺式调控元件（RSE）的npc1启动子使用启动子融合报告分析。此外，遗传功能丧失分析和体外电泳迁移率变动分析表明，蜕皮激素早期反应基因广泛复合物（br）是npc 1环腺表达的正调控的重要因素。此外，br还影响许多其他蜕皮激素生物合成基因的环腺表达，以及躯干和InR，蜕皮激素生物合成的调节中的两个关键因素。这些结果表明，蜕皮激素可能通过其早期反应基因br对发育过程中蜕皮激素的生物合成进行正反馈调节。
The growth and metamorphosis of insects are regulated by ecdysteroid hormones produced in the ring gland. Ecdysone biosynthesis-related genes are both highly and specifically expressed in the ring gland. However, the intrinsic regulation of ecdysone biosynthesis has received little attention. Here we used the Drosophila npc1 gene to study the mechanism of ring gland-specific gene expression. npc1 is important for sterol trafficking in the ring gland during ecdysone biosynthesis. We have identified a conserved ring gland-specific cis-regulatory element (RSE) in the npc1 promoter using promoter fusion reporter analysis. Furthermore, genetic loss-of-function analysis and in vitro electrophoretic mobility shift assays revealed that the ecdysone early response gene broad complex (br) is a vital factor in the positive regulation of npc1 ring gland expression. Moreover, br also affects the ring gland expression of many other ecdysone biosynthetic genes as well as torso and InR, two key factors in the regulation of ecdysone biosynthesis. These results imply that ecdysone could potentially act through its early response gene br to achieve positive feedback regulation of ecdysone biosynthesis during development.