'Rescue' of mini-genomic constructs and viruses by combinations of morbillivirus N, P and L proteins
'Rescue' of mini-genomic constructs and viruses by combinations of morbillivirus N, P and L proteins
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DOI：
10.1099/vir.0.80804-0
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发表时间：
2005-04-01
影响因子：
3.8
通讯作者：
Rima, BK
中科院分区：
文献类型：
--
作者：
Brown, DD;Collins, FM;Rima, BK
以表达麻疹病毒（MV）和牛瘟病毒（RPV）的核衣壳(N)、磷酸(P)和大(L)蛋白的辅助质粒为载体，采用标准技术对表达麻疹病毒（MV）和牛瘟病毒（RPV）负义小基因组构建物氯霉素乙酰转移酶（CAT）进行了拯救，以确定不同病毒的蛋白是否能协同作用。由来自同一病毒的N、P和L质粒组成的同质集能够从任一微型基因组构建中产生报告基因表达。除了MV N和P蛋白与RPV L蛋白结合构建MV迷你基因组或CDV N与RPV P和L结合构建RPV迷你基因组时获得低水平的活性外，来自不同病毒的异质蛋白组不具有功能。然而，只有均匀的质粒能够从全长抗基因组表达质粒中拯救感染性病毒。
Chloramphenicol acetyltransferase (CAT)-expressing negative-sense mini-genomic constructs of measles virus (MV) and rinderpest virus (RPV) were rescued by standard technology with helper plasmids expressing the nucleocapsid (N), phospho- (P) and large (L) proteins of MV, canine distemper virus (CDV) or RPV in order to determine whether the proteins of different viruses can function together. Homogeneous sets consisting of N, P and L plasmids derived from one virus were able to generate reporter gene expression from either mini-genomic construct. Heterogeneous sets of proteins from different viruses were not functional, with the exception that a low level of activity was obtained when MV N and P protein were combined with RPV L protein in the rescue of the MV mini-genomic construct, or CDV N was combined with RPV P and L in the rescue of the RPV mini-genome. However, only homogeneous sets of plasmids were able to rescue infectious virus from full-length anti-genome-expressing plasmids.